PE Mouse Anti-Human CD234 (DARC)

BD Pharmingen™ PE Mouse Anti-Human CD234 (DARC)

Name: PE Mouse Anti-Human CD234 (DARC)
Brand: BD Pharmingen™
SKU: 566424

Clone NaM185-2C3 (also known as 2C3)

(RUO)

Flow cytometric analysis of CD234 expression on human peripheral blood erythrocytes. Human peripheral blood erythrocytes were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 559320; dashed line histogram) or PE Mouse Anti-Human CD234 antibody (Cat. No. 566424; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side-light scattering characteristics of erythrocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.

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Product Details

Brand: BD Pharmingen™

Alternative Name: DUFFY; Dfy; FY; Fy glycoprotein; Glycoprotein D; GPD; CCBP1

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human CD234 Transfected Cell Line

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl

Workshop Number: VII 70537

RRID: AB_2739724

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

PE Mouse IgG1, κ Isotype Control RUO

Size 100 Tests Cat No. 559320

566424 Rev. 2

Antibody Details

NaM185-2C3

The NaM185-2C3 monoclonal antibody specifically recognizes CD234 which is also known as the Duffy antigen/receptor for chemokines (DARC), Fy glycoprotein (FY), or Glycoprotein D (GPD). CD234 is a seven-transmembrane domain acidic glycoprotein that is encoded by ACKR1 [atypical chemokine receptor 1 (Duffy blood group)] and belongs to the chemokine receptor superfamily. CD234 is responsible for the Duffy blood group system and is expressed on erythrocytes, endothelial cells, and some epithelial cells and Purkinje cells. CD234 binds to multiple chemokines including CXCL1 (also known as, MGSA or GROa), CXCL8 (IL-8), CCL2 (MCP-1), and CCL5 (RANTES). Because CD234 is not a signaling receptor, it may act as a decoy receptor or scavenger and serve to control chemokine levels. CD234 may also serve to regulate the availability of chemokines such as when it is expressed by endothelial cells at inflammatory sites or by postcapillary endothelial cells. CD234 can mediate chemokine transcytosis from the basolateral to the luminal side of the endothelium, where the bound chemokines are "presented" to regulate leucocyte trafficking. CD234 functions as a receptor for malarial parasites including Plasmodium vivax and Plasmodium knowlesi. Some individuals with mutations that prevent CD234 expression in erythrocytes are resistant to infection by these pathogens. The NaM185-2C3 antibody reportedly detects a protease-sensitive determinant and inhibits IL-8 binding to the Duffy antigen.

566424 Rev. 2

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

566424 Rev.2

Citations & References

Development References (9)

Blanchard D, Petit-Le Roux Y, Tournamille C, Folléa G. Characterization of monoclonal antibodies directed against the Fy/DARC erythrocyte glycoprotein. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:574-576. View Reference
Geoff Daniels. Duffy Blood Group System. In: Daniels G. Human blood groups : Geoff Daniels ; foreword by Ruth Sanger., 3rd ed.. Chichester, West Sussex: John Wiley & Sons; 2013:306.
Horuk R, Chitnis CE, Darbonne WC, et al. A receptor for the malarial parasite Plasmodium vivax: the erythrocyte chemokine receptor.. Science. 1993; 261(5125):1182-4. (Biology). View Reference
Nichols ME, Rubinstein P, Barnwell J, Rodriguez de Cordoba S, Rosenfield RE. A new human Duffy blood group specificity defined by a murine monoclonal antibody. Immunogenetics and association with susceptibility to Plasmodium vivax.. J Exp Med. 1987; 166(3):776-85. (Biology). View Reference
Riwom S, Janvier D, Navenot JM, Benbunan M, Muller JY, Blanchard D. Production of a new murine monoclonal antibody with Fy6 specificity and characterization of the immunopurified N-glycosylated Duffy-active molecule.. Vox Sang. 1994; 66(1):61-7. (Biology). View Reference
Van der Schoot CE, Baardman R, Lighthart P, de Jong I, Eg Von Dem Borne A, de Haas M. CD234 - DARC. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:573-574.
Walker RH, ed. Technical Manual of the American Association of Blood Banks. Arlington VA: 1990.
Wasniowska K, Petit-LeRoux Y, Tournamille C, et al. Structural characterization of the epitope recognized by the new anti-Fy6 monoclonal antibody NaM 185-2C3.. Transfus Med. 2002; 12(3):205-11. (Immunogen: Blocking, ELISA, Flow cytometry, Functional assay, Hemagglutination assay, Inhibition, Western blot). View Reference
Zola H, Swart B, Nicholson I, Voss E. CD234. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:387.

View All (9)

566424 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.