PE Mouse Anti-Human CD122

BD Pharmingen™ PE Mouse Anti-Human CD122

Name: PE Mouse Anti-Human CD122
Brand: BD Pharmingen™
SKU: 557323

Clone Mik-β2

(RUO)

Profile of anti-CD122 reactivity on peripheral blood lymphocytes of Rhesus macaque (Macaca mulatta) analyzed by flow cytometry

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Product Details

Brand: BD Pharmingen™

Alternative Name: IL-2 Receptor β chain; IL2RB; IL-2Rβ; IL15RB; P70-75

Reactivity: Rhesus, Cynomolgus, Baboon (QC Testing)

Isotype: Mouse IgG2a, κ

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

Workshop Number: V C045

Entrez Gene ID: 3560

RRID: AB_396638

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Companion Products

PE Mouse IgG2a, κ Isotype Control RUO

Size 50 Tests Cat No. 556653

557323 Rev. 5

Antibody Details

Mik-β2

Clone Mik-β2 reacts with the human form of the 75 kDa β subunit of the IL-2 receptor (IL-2Rβ). It is expressed on T cells, B cells, monocytes, myeloid precursors, NK cells and LGL. Together with the a subunit (p55, CD25) and the common γ subunit (γc subunit of the IL-2R, IL-4R, and IL-7R), the IL-2Rβ molecule forms a high-affinity, signalling receptor complex for IL-2 which can be expressed by activated T and B lymphocytes. This clone also cross-reacts with a subset of peripheral blood lymphocytes of baboon and both rhesus and cynomolgus macaque monkeys. The distribution on lymphocytes is similar to that observed with peripheral blood lymphocytes from normal human donors. A subset of CD3+ cells and a subset of CD20+ cells are CD122-positive.

557323 Rev. 5

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

557323 Rev.5

Citations & References

View product citations for antibody "557323" on CiteAb

Development References (2)

Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
Tsudo M, Kitamura F, Miyasaka M. Characterization of the interleukin 2 receptor beta chain using three distinct monoclonal antibodies. Proc Natl Acad Sci U S A. 1989; 86(6):1982-1986. (Biology). View Reference

557323 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.