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FITC Rat Anti-Mouse CD2
Product Details
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BD Pharmingen™
LFA-2; LFA-3 receptor; Ly37; Ly-37; Lymphocyte antigen 37; T11
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG2b, λ
Mouse BALB/c Thymocytes
Flow cytometry (Routinely Tested)
0.5 mg/ml
12481
AB_10896984
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553111 Rev. 14
Antibody Details
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RM2-5

The RM2-5 monoclonal antibody specifically binds to the immunoglobulin superfamily adhesion molecule, CD2 (LFA-2). CD2 is a type I transmembrane glycoprotein that serves as the the major receptor for CD48 in the mouse. CD2 is involved in T-cell activation, immunoregulation, and thymocyte maturation. In the mouse, CD2 is expressed on peripheral T lymphocytes, B lymphocytes, and NK cells, and a subpopulation of intraepithelial T lymphocytes. CD2 is expressed throughout mouse thymic ontogeny, except for distinct subsets of the CD4-CD8- early thymocytes. In the mouse bone marrow, CD2 is expressed on B220+ sIg+ CD43- pre-B cells, but not on CD43+ pro-B cells. The RM2-5 antibody is one of a set of five anti-mouse CD2 monoclonal antibodies that were classified into two groups according to their mutual competition in binding to cell surface CD2 and which block CD2-mediated cell-cell adhesion.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

553111 Rev. 14
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
553111 Rev.14
Citations & References
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Development References (16)

  1. Ayroldi E, Migliorati G, Cannarile L, Moraca R, Delfino DV, Riccardi C. CD2 rescues T cells from T-cell receptor/CD3 apoptosis: a role for the Fas/Fas-L system. Blood. 1997; 89(10):3717-3726. (Biology). View Reference
  2. Cibotti R, Punt JA, Dash KS, Sharrow SO, Singer A. Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals. Immunity. 1997; 6(3):245-255. (Clone-specific: Induction). View Reference
  3. Criado G, Feito MJ, Rojo JM. CD4-dependent and -independent association of protein tyrosine kinases to the T cell receptor/CD3 complex of CD4+ mouse T lymphocytes. Eur J Immunol. 1996; 26(6):1228-1234. (Clone-specific: Immunoprecipitation). View Reference
  4. Davis SJ, van der Merwe PA. The structure and ligand interactions of CD2: implications for T-cell function. Immunol Today. 1996; 17(4):177-187. (Biology). View Reference
  5. Hayday A, Theodoridis E, Ramsburg E, Shires J. Intraepithelial lymphocytes: exploring the Third Way in immunology. Nat Immunol. 2001; 2(11):997-1003. (Biology). View Reference
  6. Kato K, Koyanagi M, Okada H, et al. CD48 is a counter-receptor for mouse CD2 and is involved in T cell activation. J Exp Med. 1992; 176(5):1241-1249. (Biology). View Reference
  7. Kuo S, El Guindy A, Panwala CM, Hagan PM, Camerini V. Differential appearance of T cell subsets in the large and small intestine of neonatal mice. Pediatr Res. 2001; 49(4):543-551. (Biology). View Reference
  8. Masten BJ, Yates JL, Pollard Koga AM, Lipscomb MF. Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L. Am J Respir Cell Mol Biol. 1997; 16(3):335-342. (Clone-specific: Blocking). View Reference
  9. Nakamura T, Takahashi K, Fukazawa T, et al. Relative contribution of CD2 and LFA-1 to murine T and natural killer cell functions. J Immunol. 1990; 145(11):3628-3634. (Immunogen: Blocking, Inhibition). View Reference
  10. Papavasiliou F, Misulovin Z, Suh H, Nussenzweig MC. The role of Ig beta in precursor B cell transition and allelic exclusion. Science. 1995; 268(5209):408-411. (Biology). View Reference
  11. Rakasz E, Hagen M, Sandor M, Lynch RG. Gamma delta T cells of the murine vagina: T cell response in vivo in the absence of the expression of CD2 and CD28 molecules. Int Immunol. 1997; 9(1):161-167. (Biology). View Reference
  12. Rodewald HR, Awad K, Moingeon P, et al. Fc gamma RII/III and CD2 expression mark distinct subpopulations of immature CD4-CD8- murine thymocytes: in vivo developmental kinetics and T cell receptor beta chain rearrangement status. J Exp Med. 1993; 177(4):1079-1092. (Biology). View Reference
  13. Teh SJ, Killeen N, Tarakhovsky A, Littman DR, Teh HS. CD2 regulates the positive selection and function of antigen-specific CD4- CD8+ T cells. Blood. 1997; 89(4):1308-1318. (Biology). View Reference
  14. Yagita H, Asakawa J, Tansyo S, Nakamura T, Habu S, Okumura K. Expression and function of CD2 during murine thymocyte ontogeny. Eur J Immunol. 1989; 19(12):2211-2217. (Biology). View Reference
  15. Yagita H, Nakamura T, Asakawa J, et al. CD2 expression in murine B cell lineage. Int Immunol. 1989; 1(1):94-98. (Biology). View Reference
  16. Yagita H, Nakamura T, Karasuyama H, Okumura K. Monoclonal antibodies specific for murine CD2 reveal its presence on B as well as T cells. Proc Natl Acad Sci U S A. 1989; 86(2):645-649. (Immunogen: Flow cytometry). View Reference
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553111 Rev. 14

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.