Skip to main content Skip to navigation
FITC Mouse Anti-Rat RT1D
Product Details
Down Arrow Up Arrow


BD Pharmingen™
Rat (QC Testing)
Mouse BALB/c IgG1, κ
Wistar rat splenocyte membrane extract
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_393994
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
550982 Rev. 10
Antibody Details
Down Arrow Up Arrow
OX-17

The OX-17 antibody reacts with a non-polymorphic determinant of rat MHC Class II antigen, I-E equivalent. RT1D is found on peripheral B lymphocytes, thymic cortical epithelial and medullary reticular cells, epithelial cells in the adult small intestine, macrophages and dendritic cells in the intestinal lamina propria and Peyer's patches, epithelial and dendritic cells in the kidney, and activated monocytes, but not on thymocyes, erythrocytes, peripheral T cells, or brain. Its expression is upregulated on large-vessel endothelia and a variety of epithelial and glandular cells in the gastrointestinal system of rats treated with IFN-γ.

550982 Rev. 10
Format Details
Down Arrow Up Arrow
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
FITC
Blue 488 nm
494 nm
518 nm
550982 Rev.10
Citations & References
Down Arrow Up Arrow

Development References (4)

  1. Fukumoto T, McMaster WR, Williams AF, et al. Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. Eur J Immunol. 1982; 12:237-243. (Immunogen). View Reference
  2. Mayrhofer G, Pugh CW, Barclay AN. The distribution, ontogeny and origin in the rat of Ia-positive cells with dendritic morphology and of Ia antigen in epithelia, with special reference to the intestine. Eur J Immunol. 1983; 13(2):112-122. (Biology). View Reference
  3. Scriba A, Grau V, Steiniger B. Phenotype of rat monocytes during acute kidney allograft rejection: increased expression of NKR-P1 and reduction of CD43. Scand J Immunol. 1998; 47(4):332-342. (Biology). View Reference
  4. Steiniger B, Falk P, Lohmuller M, van der Meide PH. Class II MHC antigens in the rat digestive system. Normal distribution and induced expression after interferon-gamma treatment in vivo. Immunology. 1989; 68(4):507-513. (Biology). View Reference
View All (4) View Less
550982 Rev. 10

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.