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FITC Mouse Anti-Pig CD3ε
FITC Mouse Anti-Pig CD3ε

CD3ε and CD4 expression on peripheral blood leukocytes. Pig whole blood was stained with FITC-conjugated BB23-8E6-8C8 and PE- conjugated 74-12-4 (anti-pig CD4a, Cat. no. 559586) monoclonal antibodies. Erythrocytes were lysed (PharmLyse™, Cat. no. 555899), and non-viable leukocytes were excluded by staining with 7-AAD (Via-Probe™, Cat. no. 555816/555815). Flow cytometry was performed on a FACSCalibur™ (BDIS, San Jose, CA).

CD3ε and CD4 expression on peripheral blood leukocytes. Pig whole blood was stained with FITC-conjugated BB23-8E6-8C8 and PE- conjugated 74-12-4 (anti-pig CD4a, Cat. no. 559586) monoclonal antibodies. Erythrocytes were lysed (PharmLyse™, Cat. no. 555899), and non-viable leukocytes were excluded by staining with 7-AAD (Via-Probe™, Cat. no. 555816/555815). Flow cytometry was performed on a FACSCalibur™ (BDIS, San Jose, CA).

Product Details
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BD Pharmingen™
Pig (QC Testing)
Mouse BALB/c IgG2a, κ
Pig peripheral blood mononuclear cells
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_2259894
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
559582 Rev. 5
Antibody Details
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BB23-8E6-8C8

The BB23-8E6-8C8 monoclonal antibody specifically binds to the 25-kDa ε chain of the T-cell receptor-associated CD3 complex.  It recognizes all CD4+ and most CD8+ peripheral blood T lymphocytes, most thymocytes and phytohemagglutinin-stimulated blasts, and subsets of spleen and Peyer's patch lymphocytes. BB23-8E6-8C8 is a immunoglobulin isotype switch variant of the BB23-8E6 clone. This isotype-switch variant induces a proliferative response of peripheral blood mononuclear cells. The epitope recognized by BB23-8E6 mAb was designated CD3a by the Second International Swine CD Workshop.

559582 Rev. 5
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
559582 Rev.5
Citations & References
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Development References (2)

  1. Pescovitz MD, Book BK, Aasted B. Analyses of monoclonal antibodies reacting with porcine CD3: results from the Second International Swine CD Workshop. Vet Immunol Immunopathol. 1998; 60(3-4):261-268. (Clone-specific). View Reference
  2. Pescovitz MD, Book BK, Aasted B. Summary of workshop findings for antibodies reacting with porcine T-cells and activation antigens: results from the Second International Swine CD Workshop. Vet Immunol Immunopathol. 1998; 60(3-4):251-260. (Clone-specific). View Reference
559582 Rev. 5

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.