FITC Mouse Anti-Mouse IgM[a]

BD Pharmingen™ FITC Mouse Anti-Mouse IgM[a]

Name: FITC Mouse Anti-Mouse IgM[a]
Brand: BD Pharmingen™
SKU: 553516

Clone DS-1

(RUO)

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Product Details

Brand: BD Pharmingen™

Alternative Name: Igh-6a

Reactivity: Mouse (QC Testing)

Isotype: Mouse C57BL/6 IgG1, κ

Immunogen: Mouse NMRI Monoclonal Antibody TC31

Application: Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)

Concentration: 0.5 mg/ml

Entrez Gene ID: 16019

RRID: AB_394897

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

FITC Mouse IgG1, κ Isotype Control RUO

Size 0.25 mg Cat No. 550616

553516 Rev. 9

Antibody Details

DS-1

The DS-1 antibody reacts specifically with mouse IgM of Igh-C[a] and related haplotypes (e.g., BALB/c, C58, CBA, C3H/Bi, C3H/He, DBA/1, DBA/2). It does not react with IgM of Igh-C[b], Igh-C[e], or related hapltypes (e.g., C57BL, SJL, A, AKR, NZB). DS-1 antibody does not react with free µ heavy chain in vitro or in the cytoplasm of pre-B lymphocytes, which lack Ig light chain. It has not been shown to stimulate B-cell proliferation.

553516 Rev. 9

Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

553516 Rev.9

Citations & References

View product citations for antibody "553516" on CiteAb

Development References (3)

Garside P, Ingulli E, Merica RR, Johnson JG, Noelle RJ, Jenkins MK. Visualization of specific B and T lymphocyte interactions in the lymph node. Science. 1998; 281(5373):96-99. (Biology). View Reference
Sieckmann DG, Stall AM, Subbarao B. A mouse monoclonal antibody specific for an allotypic determinant of the Igha allele of murine IgM: genetic and functional analysis of Igh-6a epitopes using anti-IgM monoclonal antibodies.. Hybridoma. 1991; 10(1):121-35. (Biology). View Reference
Stall AM. Mouse immunoglobulin allotypes. In: Herzenberg LA, Weir DM, Blackwell C, ed. Weir's Handbook of Experimental Immunology. Blackwell Science Publishers; 1996:27.1-27.16.

553516 Rev. 9

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.