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FITC Mouse Anti-Human MRP1
FITC Mouse Anti-Human MRP1

Profile of human MRP1 (clone QCRL-3) reactivity on fixed, permeabilized H69AR cell line  analyzed by flow cytometry. The cell line was fixed and permeabilized with BD Cytofix/Cytoperm™ Cat. No. 554714.

Profile of human MRP1 (clone QCRL-3) reactivity on fixed, permeabilized H69AR cell line  analyzed by flow cytometry. The cell line was fixed and permeabilized with BD Cytofix/Cytoperm™ Cat. No. 554714.

Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG2a, κ
Intracellular staining (flow cytometry) (Routinely Tested)
20 µl
AB_396756
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
557593 Rev. 3
Antibody Details
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QCRL-3

Reacts with an intracellular epitope of the multidrug resistance protein (MRP1). MRP1 is a 190 kDa integral membrane phosphoglycoprotein, member of the ATP-binding cassette transporter proteins, overexpressed in some drug-selected resistant cell lines and has been shown to cause multidrug resistance in transfected cells. Clone QCRL-3 was generated using non-denatured membranes from H69AR, an MRP1-overexpressing, multidrug resistant, drug-selected cell line. Its epitope has been localized to the first nucleotide binding domain of MRP1 between amino acids 617 and 932.3 QCRL-3 does not cross-react with human MDR1 or MDR3 gene products, nor with murine MRP1. It is reported that mAb QCRL-3 inhibits the ATP-dependent transport activity of MRP1 in inside-out membrane vesicles.

557593 Rev. 3
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
557593 Rev.3
Citations & References
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Development References (4)

  1. Cole SP, Bhardwaj G, Gerlach JH, et al. Overexpression of a transporter gene in a multidrug-resistant human lung cancer cell line. Science. 1992; 258(5088):1650-1654. (Biology). View Reference
  2. Hipfner DR, Gauldie SD, Deeley RG, Cole SP. Detection of the M(r) 190,000 multidrug resistance protein, MRP, with monoclonal antibodies. Cancer Res. 1994; 54(22):5788-5792. (Biology). View Reference
  3. Hipfner DR, Mao Q, Qiu W, et al. Monoclonal antibodies that inhibit the transport function of the 190-kDa multidrug resistance protein, MRP. Localization of their epitopes to the nucleotide-binding domains of the protein. J Biol Chem. 1999; 274(22):15420-15426. (Biology). View Reference
  4. Loe DW, Almquist KC, Deeley RG, Cole SP. Multidrug resistance protein (MRP)-mediated transport of leukotriene C4 and chemotherapeutic agents in membrane vesicles. Demonstration of glutathione-dependent vincristine transport. J Biol Chem. 1996; 271(16):9675-9682. (Biology). View Reference
View All (4) View Less
557593 Rev. 3

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.