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FITC Mouse Anti-Human CD209
FITC Mouse Anti-Human CD209

Flow cytometric analysis of CD209 expression on peripheral blood monocyte-derived dendritic cells. Adherent peripheral blood mononuclear cells were cultured for 7 days with the recombinant human cytokines, Recombinant Human GM-CSF (Cat. No. 550068), Recombinant Human TNF (Cat. No. 554618) and Recombinant Human IL-4 (Cat. No. 554605). The cultured dendritic cells were harvested and stained with either FITC Mouse IgG2b, κ Isotype Control (Cat. No. 555742; dashed line histogram) or FITC Mouse Anti-Human CD209 (Cat. No. 551264/561764; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable monocyte dendritic cells.

Flow cytometric analysis of CD209 expression on peripheral blood monocyte-derived dendritic cells. Adherent peripheral blood mononuclear cells were cultured for 7 days with the recombinant human cytokines, Recombinant Human GM-CSF (Cat. No. 550068), Recombinant Human TNF (Cat. No. 554618) and Recombinant Human IL-4 (Cat. No. 554605). The cultured dendritic cells were harvested and stained with either FITC Mouse IgG2b, κ Isotype Control (Cat. No. 555742; dashed line histogram) or FITC Mouse Anti-Human CD209 (Cat. No. 551264/561764; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable monocyte dendritic cells.

Product Details
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BD Pharmingen™
DC-SIGN
Human (QC Testing)
Mouse IgG2b, κ
Human Monocyte Derived DC Cells
Flow cytometry (Routinely Tested)
20 µl
AB_394122
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
551264 Rev. 7
Antibody Details
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DCN46

The DCN46 antibody specifically binds to dendritic cell-specific ICAM-3 grabbing nonintegrin (DC-SIGN or CD209), a type-II membrane protein of approximately 44 kDa with a mannose-binding C-type lectin domain.  It is highly expressed on dendritic cells in mucosal tissues.  Its sequence is identical to the HIV-1 envelope gp120-binding C-type lectin, and reports suggest that DC-SIGN binds to HIV-1 gp120 and effectively transmits infectious HIV-1 to resting T lymphocytes expressing CD4 and chemokine receptors.  The C-type lectin domain of DC-SIGN is also capable of binding other pathogenic viruses, bacteria, and parasites.  Reports also suggest that DC-SIGN enables the highly efficient migration of dendritic cells from blood into the tissues.  It can interact with ICAM-2, which has a similar sequence as ICAM-3, and is abundantly expressed on vascular and lymphoid endothelium.  Thus, DC-SIGN mediates dendritic cells rolling and transendothelial migration, and its interaction with ICAM-2 is essential to specific migratory functions of dendritic cells.

551264 Rev. 7
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
551264 Rev.7
Citations & References
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Development References (4)

  1. Geijtenbeek TBH, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV-1 -binding protein that enhances trans-infection of T cells. Cell. 2000; 100(5):587-597. (Biology). View Reference
  2. Geijtenbeek TBH, Torensma R, van Vliet SJ, et al. Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell. 2000; 100(5):575-585. (Biology). View Reference
  3. Sallusto F, Cella M, Danieli C, Lanzavecchia A. Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartment: downregulation by cytokines and bacterial products. J Exp Med. 1995; 182(2):389-400. (Immunogen). View Reference
  4. Steinman RM. DC-SIGN: a guide to some mysteries to dendritic cells. Cell. 2000; 100(5):491-494. (Biology). View Reference
View All (4) View Less
551264 Rev. 7

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.