The KJ1-26 monoclonal antibody specifically reacts with the DO-11.10 Clonotypic T-cell Receptor (TCR) of the BALB/c-derived DO-11.10 T-cell hybridoma and T lymphocytes from the DO-11.10 transgenic mouse (TgN[DO-11.10]10Loh). The DO-11.10 TCR, an 80-90-kDa (non-reduced) or 40-44-kDa (reduced) protein, is specific for the chicken OVA(323-339)/I-A[d] complex. The DO-11.10 T-cell hybridoma also responds strongly to chicken OVA/I-A[b] and jungle fowl OVA/I-A[d] and weakly to turkey OVA/I-A[d] and I-A[b]. The DO-11.10 mouse model is valuable for studies of T-cell immigration, immunoregulation, development, activation, and function. The KJ1-26 mAb was shown to block the antigen responses of the DO-11.10 T-cell hybridoma in vitro.
The antibody was conjugated to BD Horizon BV750 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 750-nm. BD Horizon Brilliant BV750 can be excited by the violet laser (405 nm) and detected with a 750/30 nm filter with a 740 nm long pass. Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BV750 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended.