The 401901 monoclonal antibody specifically recognizes Junctional adhesion molecule-like (JAML) which is also known as Adhesion molecule interacting with CXADR antigen 1 (AMICA), or Dendritic cell-specific protein CREA7-1. JAML (AMICA) is a ~65 kDa single-pass type I transmembrane glycoprotein that is encoded by JAML which belongs to the junctional adhesion molecule (JAM) family within the Ig gene superfamily. The extracellular region of this adhesion molecule contains two Ig domains followed by a transmembrane segment and a cytoplasmic domain involved in activation signaling. JAML (AMICA) is expressed on some leucocytes including granulocytes, monocytes, γδ T cells and effector memory αβ T cells. Leucocyte JAML (AMICA) forms a homodimer that can interact with CXADR (Coxsackie virus and adenovirus receptor, also known as CAR), a cell-surface membrane receptor found in the tight junctional complexes of adjacent endothelial or epithelial cells. These heterophilic interactions can function in leucocyte activation and the regulation of leucocyte migration through epithelial and endothelial tissues, eg, during inflammatory responses or wound healing.
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor® 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.