The A12 monoclonal antibody specifically binds to CD150, a single chain type I transmembrane glycoprotein of approximately 75-95 kDa. CD150 is encoded by the SLAMF1 gene and is also known as a signaling lymphocyte activation molecule (SLAM), or IPO-3. It is expressed on T cells, B cells, thymocytes, germinal center and dendritic cells, but not on monocytes. Expression of CD150 is upregulated on T, B and dendritic cells after activation via T-cell and B-cell costimulatory molecules. It has been reported that CD150 plays a role in signal transduction.
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor® 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.