The 36-7-5 monoclonal antibody specifically reacts with the H-2K[k] MHC class I alloantigen. Cross-reactivity with splenocytes of SJL/Hsd mice has not been observed by flow cytometric analysis. Reactivity with other haplotypes (e.g., b, d, q) has not been observed.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.