The 6A6 monoclonal antibody specifically binds to mouse CD96, which is also known as T cell-activated increased late expression protein (Tactile, TACT). CD96 is a type I transmembrane glycoprotein that belongs to the Ig gene superfamily. It is variably expressed on NK cells, and T cells at all developmental stages, including thymocytes and mature αβ T cells, γδ T cells, and NKT cells. CD96 interacts with Nectin-1 (CD111) and CD155 (poliovirus receptor). CD96 can reportedly mediate adhesive interactions between NK cells or T cells and CD155+ target cells. CD96 may compete with CD226 (DNAM 1) for CD155 binding and thus limit NK cell cytokine responses.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.