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BV510 Mouse Anti-Human CD123
BV510 Mouse Anti-Human CD123

Multicolor flow cytometric analysis of CD123 expression on human peripheral blood cells. Human whole blood was stained with APC Mouse Anti-Human CD45RO (Cat. No. 559865/560899) and FITC Mouse Anti-Human CD14  (Cat. No. 555397/557153/561712) antibodies and either BD Horizon™ BV510 Mouse IgG1, k Isotype Control (Cat. No. 562946; dashed line histogram) or BD Horizon™ BV510 Mouse Anti-Human CD123 antibody (Cat. No. 563072; solid line histogram and dot plot). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two-color flow cytometric dot plot (Left Panel) shows the correlated expression of CD123 versus CD45RO for gated events with the forward and side light-scatter characteristics of viable lymphocytes. The overlapping CD123 and Ig Isotype Control fluorescence histograms (Right Panel) was derived from CD14-positive gated events (monocytes). Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

Multicolor flow cytometric analysis of CD123 expression on human peripheral blood cells. Human whole blood was stained with APC Mouse Anti-Human CD45RO (Cat. No. 559865/560899) and FITC Mouse Anti-Human CD14  (Cat. No. 555397/557153/561712) antibodies and either BD Horizon™ BV510 Mouse IgG1, k Isotype Control (Cat. No. 562946; dashed line histogram) or BD Horizon™ BV510 Mouse Anti-Human CD123 antibody (Cat. No. 563072; solid line histogram and dot plot). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two-color flow cytometric dot plot (Left Panel) shows the correlated expression of CD123 versus CD45RO for gated events with the forward and side light-scatter characteristics of viable lymphocytes. The overlapping CD123 and Ig Isotype Control fluorescence histograms (Right Panel) was derived from CD14-positive gated events (monocytes). Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

Product Details
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BD Horizon™
IL3RA; IL-3RA; IL-3Rα; IL-3R-alpha; Interleukin-3 receptor subunit alpha
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human IL-3Rα Transfected Cell Line
Flow cytometry (Routinely Tested)
5 µl
VI C-67
3563
AB_2728102
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Brilliant Violet™ 510 is a trademark of Sirigen.
563072 Rev. 1
Antibody Details
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9F5

The 9F5 monoclonal antibody specifically binds to CD123. CD123 is the 70 kDa IL-3 receptor α chain (IL-3Rα) that associates with the 120-140 kDa β subunit (CD131/Common β-chain/βc) to form the functional IL-3 receptor complex. The βc chain is also shared with distinct α chain subunits to form the functional heterodimeric receptors for interleukins IL-5 and GM-CSF. IL-3Rα is expressed on a subset of peripheral blood dendritic cells, myeloid precursors, basophils, mast cells, macrophages, and megakaryocytes. Reports indicate that IL-3Rα is also expressed on lymphocytes. The IL-3R plays an important role in hematopoietic progenitor cell growth and differentiation. This antibody does not block binding of IL-3 to the IL-3 receptor.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.

563072 Rev. 1
Format Details
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BV510
The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV510
Violet 405 nm
327 nm, 405 nm
512 nm
563072 Rev.1
Citations & References
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Development References (5)

  1. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
  2. Korpelainen EI, Gamble JR, Smith WB, et al. The receptor for interleukin 3 is selectively induced in human endothelial cells by tumor necrosis factor alpha and potentiates interleukin 8 secretion and neutrophil transmigration.. Proc Natl Acad Sci USA. 1993; 90(23):11137-41. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
  3. Macardle PJ, Chen Z, Shih CY, et al. Characterization of human leucocytes bearing the IL-3 receptor. Cell Immunol. 1996; 168(1):59-68. (Clone-specific: Flow cytometry). View Reference
  4. Smith WB, Guida L, Sun Q, et al. Neutrophils activated by granulocyte-macrophage colony-stimulating factor express receptors for interleukin-3 which mediate class II expression. Blood. 1995; 86(10):3938-3944. (Clone-specific: Flow cytometry). View Reference
  5. Sun Q, Woodcock JM, Rapoport A, et al. Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist.. Blood. 1996; 87(1):83-92. (Clone-specific: Immunoprecipitation, Western blot). View Reference
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563072 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.