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BV480 Mouse Anti-Human MICA
Product Details
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BD OptiBuild™
MHC class I chain-related protein A; MHC class I related chain A; MIC-A; PERB11.1; stress inducible class I homolog
Human (Tested in Development)
Mouse IgG2b, κ
Human MICA Transfected Cell Line
Flow cytometry (Qualified)
0.2 mg/ml
AB_2874020
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV480 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 480 is covered by one or more of the following US patents: 8,575,303; 8,354,239.
749766 Rev. 3
Antibody Details
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159227

The 159227 monoclonal antibody specifically recognizes MHC class I polypeptide-related sequence A (MICA) which is also known as MIC-A, MHC class I chain-related protein A, or stress inducible class I homolog. This ~70 kDa single-pass type I transmembrane glycoprotein is encoded by MICA (major histocompatibility complex class I chain-related gene A) which is a highly polymorphic gene. MICA is very homologous to another protein, MICB. Both MICA and MICB are homologous to major histocompatibility complex (MHC) class I molecules although they lack association with β2 microglobulin. MICA and MICB each contain three extracellular Ig domains but have no capacity to bind peptides. MICA is expressed on some gut epithelial cells. Its expression by other epithelial cells and cell types, including fibroblasts and endothelial cells, is induced by stress, eg, stress caused by bacterial and viral infections, autoimmunity, transplantation rejection, or cellular transformation. MICA and MICB are ligands for NKG2D (CD314), an activating receptor expressed by natural killer (NK) cells, γδ T cells, CD8+ αβ T cells, and some CD4+ αβ T cells.

The antibody was conjugated to BD Horizon BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436-nm and Em Max at 478-nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon BV510 (525/40-nm) filter set.  BV480 has less spillover into the BV605 detector and, in general, is brighter than BV510.

749766 Rev. 3
Format Details
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BV480
The BD Horizon Brilliant Violet™ 480 (BV480) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology fluorochrome has an excitation maximum (Ex Max) of 440-nm and an emission maximum (Em Max) of 479-nm. Driven by BD innovation, BV480 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 480-nm (e.g., a 525/50 bandpass filter). The increased fluorescence intensity of BV480 and narrower emission spectra, make it a good alternative for BV510 or V500. Due to its excitation profile, BV480 will also has less cross-laser excitation with the UV laser, resulting in less spillover into UV channels compared to BV510. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV480
Violet 405 nm
440 nm
479 nm
749766 Rev.3
Citations & References
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Development References (3)

  1. Gómez-Lomelí P, Bravo-Cuellar A, Hernández-Flores G, et al. Increase of IFN-γ and TNF-α production in CD107a + NK-92 cells co-cultured with cervical cancer cell lines pre-treated with the HO-1 inhibitor.. Cancer Cell Int. 2014; 14(1):100. (Clone-specific: Flow cytometry). View Reference
  2. Niu C, Jin H, Li M, et al. Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and γδ T cell-mediated lysis in multiple myeloma.. Oncotarget. 2017; 8(4):5954-5964. (Clone-specific: Flow cytometry). View Reference
  3. Robinet P, Baychelier F, Fontaine T, et al. A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.. J Immunol. 2014; 192(11):5332-42. (Clone-specific: Flow cytometry). View Reference
749766 Rev. 3

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.