The 90220 monoclonal antibody specifically binds to the beta subunit (IL-10R beta/IL-10RB/IL-10Rβ) of the heteromeric IL-10 Receptor complex. This type I transmembrane glycoprotein is also known as, subunit 2 (IL-10R2) or CD210b. The IL-10 Receptor complex is formed by the binding of dimeric IL-10 to two cell surface alpha subunits of the IL-10 Receptor (CD210a/IL-10RA/IL-10Rα). This complex can then recruit two CD210b subunits resulting in the transduction of intracellular JAK/STAT pathway signals. CD210b is also a component of cytokine receptor complexes specific for IL-22, IL-26, IL-28 and IL-29. CD210b is expressed by a variety of cell types including T cells, B cells, NK cells, monocytes and macrophages.
The antibody was conjugated to BD Horizon BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436-nm and Em Max at 478-nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon BV510 (525/40-nm) filter set. BV480 has less spillover into the BV605 detector and, in general, is brighter than BV510.