Skip to main content Skip to navigation
BV421 Rat Anti-Mouse CD369 (Clec7a)
BV421 Rat Anti-Mouse CD369 (Clec7a)

Flow cytometric analysis of CD369 (Clec7a) expression on mouse peripheral blood leucocytes (PBL) and peritoneal exudate cells (PEC).  

PBL: BALB/c mouse PBL were stained with FITC Rat Anti-Mouse Ly-6G antibody (Cat. No. 551460 or 561105) and either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; Plot A) or BD OptiBuild™ BV421 Rat Anti-Mouse CD369 (Clec7a) antibody (Cat. No. 749795; Plot B) at 1 μg/test. Two-parameter pseudocolor density plots showing the correlated expression of CD369 (Clec7a) [or Ig Isotype control staining] versus Ly-6G were derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations.

PEC: BALB/c mouse thioglycolate-elicited PEC were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141 or 553142). The cells were then stained with Alexa Fluor® 647 Rat Anti-Mouse F4/80 antibody (Cat. No. 565854) and either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; Plot C) or BD OptiBuild™ BV421 Rat Anti-Mouse CD369 (Clec7a) antibody (Cat. No. 749795; PlotD) at 1 μg/test. Two-parameter pseudocolor density plots showing the correlated expression of CD369 (Clec7a) [or Ig Isotype control] versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable PEC.

Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo® software. Data shown in this Technical Data Sheet are qualification data only and do not represent a specific OptiBuild™ lot.

Flow cytometric analysis of CD369 (Clec7a) expression on mouse peripheral blood leucocytes (PBL) and peritoneal exudate cells (PEC).  

PBL: BALB/c mouse PBL were stained with FITC Rat Anti-Mouse Ly-6G antibody (Cat. No. 551460 or 561105) and either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; Plot A) or BD OptiBuild™ BV421 Rat Anti-Mouse CD369 (Clec7a) antibody (Cat. No. 749795; Plot B) at 1 μg/test. Two-parameter pseudocolor density plots showing the correlated expression of CD369 (Clec7a) [or Ig Isotype control staining] versus Ly-6G were derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations.

PEC: BALB/c mouse thioglycolate-elicited PEC were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141 or 553142). The cells were then stained with Alexa Fluor® 647 Rat Anti-Mouse F4/80 antibody (Cat. No. 565854) and either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; Plot C) or BD OptiBuild™ BV421 Rat Anti-Mouse CD369 (Clec7a) antibody (Cat. No. 749795; PlotD) at 1 μg/test. Two-parameter pseudocolor density plots showing the correlated expression of CD369 (Clec7a) [or Ig Isotype control] versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable PEC.

Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo® software. Data shown in this Technical Data Sheet are qualification data only and do not represent a specific OptiBuild™ lot.

Product Details
Down Arrow Up Arrow


BD OptiBuild™
Dectin1; Dectin-1; DC-associated C-type lectin 1; BGR; Beta-glucan receptor
Mouse (Tested in Development)
Rat IgG2a, κ
Mouse Dectin-1/Clec7a Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
AB_2874049
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  10. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
749795 Rev. 4
Antibody Details
Down Arrow Up Arrow
218820

The 218820 monoclonal antibody specifically recognizes CD369 which is also known as Dendritic cell-associated C-type lectin 1 (DC-associated C-type lectin 1, Dectin-1, or Dectin1), or Beta-glucan receptor (Bgr). CD369 is a ~ 43 kDa type II transmembrane C-type lectin that is encoded by Clec7a (C-type lectin domain family 7 member a). CD369 (Clec7a) contains a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain, a transmembrane segment, and a cytoplasmic tail with an immunoreceptor tyrosine-based activation motif (ITAM). It is expressed on monocytes/macrophages, neutrophils, and some populations of dendritic cells. CD369 (Clec7a) binds to beta-glucan polymers and functions as a pattern recognition receptor (PRR) in innate immune response to fungal and bacterial pathogens. CD369 (Clec7a)-mediated signaling may play roles in leucocyte responses including phagocytosis or enhanced cytokine production.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

749795 Rev. 4
Format Details
Down Arrow Up Arrow
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
BV421
Violet 405 nm
407 nm
423 nm
749795 Rev.4
Citations & References
Down Arrow Up Arrow

Development References (3)

  1. Ariizumi K, Shen GL, Shikano S, et al. Identification of a novel, dendritic cell-associated molecule, dectin-1, by subtractive cDNA cloning.. J Biol Chem. 2000; 275(26):20157-67. (Biology). View Reference
  2. Saijo S, Fujikado N, Furuta T, et al. Dectin-1 is required for host defense against Pneumocystis carinii but not against Candida albicans.. Nat Immunol. 2007; 8(1):39-46. (Biology). View Reference
  3. Taylor PR, Brown GD, Reid DM, et al. The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages.. J Immunol. 2002; 169(7):3876-82. (Biology). View Reference
749795 Rev. 4

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.