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BV421 Rat Anti-Mouse CD161f
BV421 Rat Anti-Mouse CD161f
Two-color flow cytometric analysis of Mouse CD161f expression on C57BL/6 mouse splenocytes. Mouse splenic leucocytes were stained with APC Mouse Anti-Mouse NK1.1 antibody (Cat. No. 550627/561117) and either BD Horizon™ BV421 Rat IgG1, κ Isotype Control (Cat. No. 562868; Left Plot) or BD Horizon BV421 Rat Anti-Mouse CD161f antibody (Cat. No. 566304; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of NK1.1 versus CD161f (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Two-color flow cytometric analysis of Mouse CD161f expression on C57BL/6 mouse splenocytes. Mouse splenic leucocytes were stained with APC Mouse Anti-Mouse NK1.1 antibody (Cat. No. 550627/561117) and either BD Horizon™ BV421 Rat IgG1, κ Isotype Control (Cat. No. 562868; Left Plot) or BD Horizon BV421 Rat Anti-Mouse CD161f antibody (Cat. No. 566304; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of NK1.1 versus CD161f (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Product Details
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BD Horizon™
Klrb1f; Nkrp1-f; Nkrp1f; NKR-P1F
Mouse (QC Testing)
Rat PVG, also known as HO, Black Hooded IgG1, κ
Mouse NKRP1F Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2869747
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566304 Rev. 1
Antibody Details
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C8

The C8 monoclonal antibody specifically recognizes C57BL/6 mouse CD161 antigen-like family member F (CD161f ). CD161f is also known as Natural killer cell surface protein NKR-P1F (Nkrp1f), and is encoded by Klrb1f  (Killer cell lectin-like receptor subfamily B member 1F). CD161f is expressed on natural killer (NK) cells, and some dendritic cells and activated lymphocytes. CD161f  binds to C-type lectin related protein c, d, and g (Clr-c, d, g) which are expressed on several cell types including dendritic cells, B cells, and activated T cells. CD161f-mediated signaling can reportedly costimulate T cell proliferation and IL-2 production, and may play a role in dendritic cell maturation.

566304 Rev. 1
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
566304 Rev.1
Citations & References
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Development References (9)

  1. Aust JG1, Gays F, Mickiewicz KM, Buchanan E, Brooks CG.. The expression and function of the NKRP1 receptor family in C57BL/6 mice.. J Immunol. 2009; 183(1):106-116. (Immunogen: Flow cytometry). View Reference
  2. Carlyle JR, Mesci A, Ljutic B, et al. Molecular and genetic basis for strain-dependent NK1.1 alloreactivity of mouse NK cells.. J Immunol. 2006; 176(12):7511-24. (Biology). View Reference
  3. Chai L, Wu S, Liu G, Wang Z, Tian W, Ma Y. OCILRP2 signaling synergizes with LPS to induce the maturation and differentiation of murine dendritic cells.. Biochem Biophys Res Commun. 2014; 446(4):836-42. (Biology). View Reference
  4. Iizuka K, Naidenko OV, Plougastel BF, Fremont DH, Yokoyama WM. Genetically linked C-type lectin-related ligands for the NKRP1 family of natural killer cell receptors.. Nat Immunol. 2003; 4(8):801-7. (Biology). View Reference
  5. Kirkham CL, Carlyle JR. Complexity and Diversity of the NKR-P1:Clr (Klrb1:Clec2) Recognition Systems. Front Biosci. 2014; 5(5):1-16. (Biology). View Reference
  6. Plougastel B, Matsumoto K, Dubbelde C, Yokoyama WM. Analysis of a 1-Mb BAC contig overlapping the mouse Nkrp1 cluster of genes: cloning of three new Nkrp1 members, Nkrp1d, Nkrp1e, and Nkrp1f.. Immunogenetics. 2001; 53(7):592-8. (Biology). View Reference
  7. Rozbeský D, Ivanova L, Hernychová L, Grobárová V, Novák P, Černý J. Nkrp1 family, from lectins to protein interacting molecules.. Molecules. 2015; 20(2):3463-78. (Clone-specific). View Reference
  8. Tian W, Feng B, Liou HC. Silencing OCILRP2 leads to intrinsic defects in T cells in response to antigenic stimulation.. Cell Immunol. 2005; 235(1):72-84. (Biology). View Reference
  9. Tian W, Nunez R, Cheng S, et al. C-type lectin OCILRP2/Clr-g and its ligand NKRP1f costimulate T cell proliferation and IL-2 production.. Cell Immunol. 2005; 234(1):39-53. (Biology). View Reference
View All (9) View Less
566304 Rev. 1

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