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BV421 Mouse Anti-Human CD245
Product Details
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BD OptiBuild™
MYO18A; myosin 18A; unconventional myosin-XVIIIa; MAJN; MYSPDZ
Human (Tested in Development)
Mouse BALB/c IgG1, κ
Human NK Cell Line
Flow cytometry (Qualified)
0.2 mg/ml
VI 6T-021; VII 70008; X 10-43
AB_2874958
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

  1. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  2. Researchers should determine the optimal concentration of this reagent for their individual applications.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  7. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  9. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  10. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
750844 Rev. 2
Antibody Details
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DY12

The DY12 monoclonal antibody specifically recognizes CD245 which is also known as p220/240. CD245 is variably expressed by T cells, B cells. NK cells, granulocytes, and platelets and more highly expressed on monocytes. CD245 expression can be upregulated on activated T cells, B cells, and NK cells. CD245 has been identified as Myosin 18A that is encoded by MYO18A (Myosin XVIIIA). Myosin 18A is also known as Myosin containing a PDZ domain (MysPDZ), Molecule associated with JAK3 N-terminus (MAJN), and Surfactant protein receptor SP-R2101 (SP-R2101). Myosin 18A is involved in intracellular transport processes and can also reportedly serve as a cell surface receptor for Surfactant Protein A (SP-A). The DY12 antibody reportedly immunoprecipitated long α (230 kDa) and short β (190 kDa) isoforms of Myosin 18A expressed by normal human lung extracts and by the YT2C2 NK cell line-further evidence suggested that the Myosin 18Aα isoform is expressed at the cell surface. CD245 stimulation mediated by bound and crosslinked DY12 antibody or the SP-A ligand can reportedly augment NK cell degranulation or IL-2-activated NK cell-mediated cytotoxicity against certain tumor target cells. Moreover, stimulation through CD245 can upregulate CD137/4-1BB expression on NK cells and promote NK cell-mediated cytotoxicity against CD137 Ligand-expressing target cells.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

750844 Rev. 2
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
750844 Rev.2
Citations & References
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Development References (5)

  1. Boumsell L, Nikolova M, Marie-Cardine A, Bensussan A. CD245 Workshop report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:691-692.
  2. De Masson A, Giustiniani J, Marie-Cardine A, et al. Identification of CD245 as myosin 18A, a receptor for surfactant A: A novel pathway for activating human NK lymphocytes.. Oncoimmunology. 2016; 5(5):e1127493. (Immunogen: Flow cytometry, Functional assay, Immunohistochemistry). View Reference
  3. Gurka S, Dirks S, Photiadis J, Kroczek RA. Expression analysis of surface molecules on human thymic dendritic cells with the 10th HLDA Workshop antibody panel.. Clin Transl Immunology. 2015; 4(10):e47. (Clone-specific: Flow cytometry). View Reference
  4. Okumura K, Kato K, Kobata T. T-cell antigens: Section report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:23-32.
  5. Zola H, Swart B, Boumsell L, Mason DY. Human Leucocyte Differentiation Antigen nomenclature: update on CD nomenclature. Report of IUIS/WHO Subcommittee.. J Immunol Methods. 2003; 275(1-2):1-8. (Clone-specific). View Reference
View All (5) View Less
750844 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.