BUV805 Mouse Anti-Mouse I-Ak

BD OptiBuild™ BUV805 Mouse Anti-Mouse I-Ak

Name: BUV805 Mouse Anti-Mouse I-Ak
Brand: BUV805 Mouse Anti-Mouse I-Ak
SKU: 748855

Clone 10-3.6 (RUO)

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Catalog No:748855

Unit:EA (1 Each)

Size:

50 µg

Product Details

Brand: BD OptiBuild™

Alternative Name: Aβk

Reactivity: Mouse (Tested in Development)

Isotype: Mouse CWB IgG2a, κ

Immunogen: C3H mouse splenocytes

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

RRID: AB_2873258

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV805 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Data Sheets

Technical Data Sheet (TDS)

Safety Data Sheet

748855 Rev. 2

Antibody Details

10-3.6

The 10-3.6 monoclonal antibody specifically recognizes the β chain of the I-A[k] MHC class II alloantigen. It cross-reacts with splenocytes from mice of the H-2[f], H-2[r], and H-2[s] haplotypes, from nonobese diabetic (NOD, H-2[g7]) mice, and with lymph node cells of BN, DA, WRA, and WRC rats. Reactivity with other haplotypes (e.g., b, d, p, q) has not been observed. Long-term in vivo treatment with 10-3.6 mAb has been reported to prevent the spontaneous development of autoimmune insulin-dependent diabetes mellitus (IDDM) in female NOD mice.

The antibody was conjugated to BD Horizon™ BUV805 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348 nm and an acceptor dye with an Em Max at 805 nm. BD Horizon Brilliant BUV805 can be excited by the ultraviolet laser (355 nm) and detected with a 820/60 filter and a 770LP.

748855 Rev. 2

Format Details

BUV805

The BD Horizon Brilliant™ Ultraviolet 805 (BUV805) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This tandem fluorochrome is comprised of a BUV395 donor with an excitation maximum (Ex Max) of 351-nm and an acceptor dye with an emission maximum (Em Max) at 803-nm. BUV805, driven by BD innovation, is designed to be excited by the ultraviolet laser (355 nm) and detected using an optical filter centered near 805-nm (e.g., a 820/60 or a 780/60 bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: BUV805

Excitation Max: 351 nm

Emission Max: 803 nm

748855 Rev.2

Citations & References

No Citations Are Available for this Product

748855 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.