The 235614 monoclonal antibody specifically binds to CRACC (CD2-like receptor activating cytotoxic cells) which is also known as CD319, CS1 (CD2 subset 1), SLAMF7, 19A, and 19A24. CRACC is a type I transmembrane glycoprotein that belongs to the CD2 family of receptors within the Ig superfamily. CRACC is expressed on most NK cells and subsets of CD8+ T cells, CD4+ T cells, B cells, macrophages, and dendritic cells. CRACC reportedly plays roles in the activation, proliferation and effector functions of T cells, NK cells, and other leucocytes.
The antibody was conjugated to BD Horizon™ BUV805 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348 nm and an acceptor dye with an Em Max at 805 nm. BD Horizon Brilliant BUV805 can be excited by the ultraviolet laser (355 nm) and detected with a 820/60 filter and a 770LP.