The TX45 monoclonal antibody recognizes human CD300c which is also known as Leukocyte immunoglobulin-like receptor (LIR), CMRF35-like molecule 6 (CLM-6), or Immunoglobulin superfamily member 16 (IGSF16). CD300c is a type I transmembrane glycoprotein that belongs to the CMRF family within the Ig superfamily. CD300c is primarily expressed on myeloid cells and has also been detected on some T cells, B cells, and NK cells. Amongst freshly-isolated leucocytes, the TX45 antibody only detects CD300c expressed on monocytes. CD300c is involved in the regulation of proinflammatory cytokine production by leucocytes.
The antibody was conjugated to BD Horizon™ BUV805 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348 nm and an acceptor dye with an Em Max at 805 nm. BD Horizon Brilliant BUV805 can be excited by the ultraviolet laser (355 nm) and detected with a 820/60 filter and a 770LP.