The 2D3 monoclonal antibody specifically binds to CD249. CD249 is encoded by ENPEP and is also known as Aminopeptidase A (APA/AP-A), Glutamyl aminopeptidase (EAP), and gp160. CD249 is expressed by epithelial and endothelial cells in a variety of tissues including those that comprise the proximal tubules and glomeruli of the kidneys. It is also expressed by early B-lineage cells and cell lines. CD249 belongs to the peptidase M1 family and catalyzes the release of N-terminal glutamate (and to a lesser degree, aspartate) from peptides. This ectoenzyme catalyzes the conversion of angiotensin II to angiotensin III which is important in the local regulation of blood pressure. It may also play roles in the regulation of angiogenesis and development of B cells.
The antibody was conjugated to BD Horizon™ BUV805 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348 nm and an acceptor dye with an Em Max at 805 nm. BD Horizon Brilliant BUV805 can be excited by the ultraviolet laser (355 nm) and detected with a 820/60 filter and a 770LP.