The MMRI-7 monoclonal antibody specifically recognizes CD205 which is also known as DEC-205, Lymphocyte antigen 75 (Ly-75), or C-type lectin domain family 13 member B (CLEC13B). CD205 is a ~205 kDa type I transmembrane glycoprotein with C-type lectin external domains. DEC-205 is expressed on myeloid and plasmacytoid dendritic cells, thymic epithelial cells, and at lower levels on monocytes, T cells, B cells, and natural killer (NK) cells. DEC-205 likely functions as an endocytic receptor and is involved in antigen uptake and processing.
The antibody was conjugated to BD Horizon™ BUV805 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348 nm and an acceptor dye with an Em Max at 805 nm. BD Horizon Brilliant BUV805 can be excited by the ultraviolet laser (355 nm) and detected with a 820/60 filter and a 770LP.