The 6C6 monoclonal antibody specifically binds to CD182. CD182 is also known as the interleukin-8 receptor B (IL-8RB), a G-protein-linked seven transmembrane domain protein, expressed on monocytes and granulocytes. It is also expressed on a subset of T cells. Both IL-8RA (CXCR1) and IL-8RB (CXCR2) are high affinity receptors for IL-8. IL-8 is an inflammatory cytokine which induces chemotaxis of neutrophils, basophils and T lymphocytes.
The antibody was conjugated to BD Horizon™ BUV805 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348 nm and an acceptor dye with an Em Max at 805 nm. BD Horizon Brilliant BUV805 can be excited by the ultraviolet laser (355 nm) and detected with a 820/60 filter and a 770LP.