The V7.1 monoclonal antibody specifically recognizes CD101 which is also known as P126 or Glu-Trp-Ile EWI motif-containing protein 101 (EWI-101). CD101 is encoded by IGSF2 (Immunoglobulin superfamily member 2) and belongs to the EWI (sharing a conserved glutamine-tryptophan-isoleucine motif) family within the Ig superfamily. CD101 is a ~135-140 kDa type I transmembrane glycoprotein that has a short cytoplasmic domain with potential phosphorylation sites and an extracellular region with seven immunoglobulin-variable (IgV)-like domains. CD101 is expressed as homodimers by granulocytes, monocytes, dendritic cells, and some T cells. Its expression by T cells may be upregulated upon activation through the CD3 complex. CD101 may play a role in TCR/CD3-dependent T-cell activation.
The antibody was conjugated to BD Horizon™ BUV805 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348 nm and an acceptor dye with an Em Max at 805 nm. BD Horizon Brilliant BUV805 can be excited by the ultraviolet laser (355 nm) and detected with a 820/60 filter and a 770LP.