The MEM-78 monoclonal antibody specifically recognizes CD10. CD10 is a 100 kDa type II transmembrane, glycosylated, zinc-containing metalloprotease. The CD10 antigen is also known as common acute lymphoblastic leukemia antigen (CALLA), neutral endopeptidase (NEP), gp100, and enkephalinase. The CD10 antigen is found on lymphocytes from samples with acute B-lymphoid leukemia. The CD10 antigen is also present on a wide variety of normal and neoplastic cell types including renal epithelia, fibroblasts, granulocytes, germinal center B lymphocytes, neutrophils, some T-cell leukemias, and some lymphoma, melanoma, and glioma cell lines. The CD10 antigen cleaves a number of biologically active peptides, including fMLP, and may modulate the chemotactic activity of fMLP towards neutrophils. Inhibition of the CD10 antigen promotes B-cell maturation, suggesting that it plays a role in B-cell development.
The antibody was conjugated to BD Horizon™ BUV805 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348 nm and an acceptor dye with an Em Max at 805 nm. BD Horizon Brilliant BUV805 can be excited by the ultraviolet laser (355 nm) and detected with a 820/60 filter and a 770LP.