The Zt/g4 monoclonal antibody specifically binds to RON, which is also known as CD136, Macrophage-stimulating protein receptor (MSP-Receptor, MSP-R), Macrophage stimulating 1 receptor (c-met-related tyrosine kinase) (MST1R), or Protein-tyrosine kinase 8 (PTK8). RON is a type I transmembrane glycoprotein that belongs to the receptor tyrosine kinase family. It is expressed on cells as a disulfide-linked heterodimer. RON is expressed on macrophages, keratinocytes, erythroblasts and epithelial cells from the skin, lung, kidney, liver, intestine, colon, and bone marrow. RON reportedly regulates many physiological processes including the survival, migration, differentiation, and effector functions of a variety of cell types including epithelial cells, and macrophages. RON binds Macrophage stimulating protein (MSP). Overexpression of RON may contribute to the malignant transformation of epithelial cells.
The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm. BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor® 700-like dyes (eg, 712/20-nm filter).
Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV737 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV737 reagents (eg, CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.