The LS132.1D9 (aka, 1D9) monoclonal antibody specifically recognizes C-C chemokine receptor type 2 (CCR2 or CC-CKR-2) which is also known as CD192, CKR2, CMKBR2, or Monocyte chemoattractant protein 1 receptor (MCP-1 Receptor or MCP-1-R). CCR2 (CD192) is a seven-transmembrane, G-protein-coupled, glycoprotein receptor that belongs to the beta chemokine receptor family. It is expressed on basophils, monocytes/macrophages, dendritic cells, activated T cells and B cells. CCR2 (CD192) serves as a receptor for Monocyte chemoattractant protein 1 (MCP-1/CCL2), MCP-2/CCL8, MCP-3/CCL7, and MCP-4/CCL13. CD192 exists in two forms, CD192A and CD192B. The two forms are derived from alternatively spliced variants of a single gene and differ at their intracellular C-terminal ends. CD192 plays an important role in inflammatory responses including monocytic infiltration of tissues associated with certain diseases, eg, atherosclerosis, rheumatoid arthritis, and tumors.
The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm. BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor® 700-like dyes (eg, 712/20-nm filter).
Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV737 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV737 reagents (eg, CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.