The 16.1 monoclonal antibody recognizes the C57BL/6 mouse CD161a, CD161b, and CD161c (CD161a, b, c) cell surface molecules, which are also known as Natural killer cell receptor P1A (NKRP1A), NKRP1D, and NKRP1C, respectively. These cell surface molecules are expressed by natural killer (NK) cells and may play a role in NK cell functions. The 16.1 antibody does not crossreact with CD161f (NKRP1F). This antibody likely binds to CD161 molecules expressed by lymphoid cells from some other mouse strains.
The antibody was conjugated to BD Horizon™ BUV661 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 661-nm. BD Horizon Brilliant BUV661 can be excited by the ultraviolet laser (355 nm) and detected with a 670/25 filter and a 630 nm LP. Due to cross laser excitation of this dye, there may be significant spillover into channels detecting APC-like emissions (eg, 670/25-nm filter).
Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV661 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV661 reagents (eg, CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone-specific compensation controls when using these reagents.