The AMS 9.1 antibody reacts specifically with the Fc portion of mouse IgD of Igh C[a] haplotype (eg, AKR, BALB/c, CBA, C3H/He, C58, DBA/1, DBA/2, NZB). It does not react with IgD of Igh-C[b] or Igh-C[e] haplotypes (eg, C57BL/6, C57BL/10, SJL, A). AMS 9.1 mAb is effective for detection of cell-surface Ig by immunofluorescent staining with flow cytometric analysis. It has been reported to stimulate B-cell proliferation.
The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP. Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).