The CerCLIP monoclonal antibody specifically recognizes the class II associated invariant chain peptides (CLIP). CLIP is the remaining segment of class II invariant chain (Ii) after proteolytic degradation which stays associated with HLA-DR and represents a final intermediate in the removal of invariant chain from class II molecules during antigen processing. The removal of CLIP and subsequent loading of the antigenic peptide is facilitated by the non-classical class II molecule HLA-DM. CLIP can be detected, in association with HLA-DR, on the surface of T2DR3, a mutant cell line lacking HLA-DM.
The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP. Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).