Reacts with platelet-endothelial cell tetraspan antigen-3 (PETA-3), a 27 kD membrane glycoprotein, expressed on platelets, megakaryocytes, lymphocytes (weak), monocytes, endothelial cells and epithelial cells. PETA-3 (CD151) associates with β1 integrin in certain tissues. This has also been shown with other tetraspan superfamily members, like CD9, CD63 and α5β1. Reports indicate that this association or colocalization of CD151 with β1 integrin in tissues suggests a functional role of this molecule, however, this role has not been elucidated yet. It has also been reported that antibody 14A2.H1 is capable of platelet activation in vitro. Studies showed that different clones of CD151 monoclonal antibodies display strikingly different patterns of binding to human haemopoietic cells and tissue sections, and that this is due at least in part to the presence of the protein in complexes with different integrins.
The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP. Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).