The M-L13 monoclonal antibody specifically binds to a 24 kDa type III transmembrane protein that is expressed on platelets, pre-B cells, monocytes, endothelial and epithelial cells. CD9 belongs to a family of membrane proteins called tetraspanins that transverse the membrane four times. CD9 is weakly expressed on resting mature B cells. M-L13 induces platelet aggregation and activation. This antibody is also suitable for staining acetone-fixed, frozen tissue sections.
The antibody was conjugated to BD Horizon™ BUV563 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 which has an Ex Max of 348 nm and an acceptor dye. The tandem has an Em Max at 563 nm. BD Horizon BUV563 can be excited by the 355 nm ultraviolet laser. On instruments with a 561 nm Yellow-Green laser, the recommended bandpass filter is 585/15 nm with a 535 nm long pass to minimize laser light leakage. When BD Horizon BUV563 is used with an instrument that does not have a 561 nm laser, a 560/40 nm filter with a 535 nm long pass may be more optimal. Due to the excitation and emission characteristics of the acceptor dye, there may be spillover into the PE and PE-CF594 detectors. However, the spillover can be corrected through compensation as with any other dye combination.