The L60 monoclonal antibody specifically binds to CD43 which is also known as Leukosialin (LSN) or Galactoglycoprotein (GALGP). CD43 is a ~95-135 kDa heavily O-sialylated type I transmembrane glycoprotein that is encoded by SPN (sialophorin) and belongs to the cell surface mucin family. The L60 antibody recognizes a sialic acid-dependent determinant on CD43. CD43 is highly expressed on T lymphocytes, thymocytes, monocytes, granulocytes, bone marrow stem cells, pre-B cells and activated B cells plasma cells but not on resting peripheral blood B cells, red blood cells, and non-hematopoietic cells. CD43 is enzymatically shed from leucocyte surfaces following activation by various stimuli. CD43 appears to be involved in mediating intercellular interactions that regulate leucocyte functions.
The antibody was conjugated to BD Horizon™ BUV563 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 which has an Ex Max of 348 nm and an acceptor dye. The tandem has an Em Max at 563 nm. BD Horizon BUV563 can be excited by the 355 nm ultraviolet laser. On instruments with a 561 nm Yellow-Green laser, the recommended bandpass filter is 585/15 nm with a 535 nm long pass to minimize laser light leakage. When BD Horizon BUV563 is used with an instrument that does not have a 561 nm laser, a 560/40 nm filter with a 535 nm long pass may be more optimal. Due to the excitation and emission characteristics of the acceptor dye, there may be spillover into the PE and PE-CF594 detectors. However, the spillover can be corrected through compensation as with any other dye combination.