The LS132.1D9 (aka, 1D9) monoclonal antibody specifically recognizes C-C chemokine receptor type 2 (CCR2 or CC-CKR-2) which is also known as CD192, CKR2, CMKBR2, or Monocyte chemoattractant protein 1 receptor (MCP-1 Receptor or MCP-1-R). CCR2 (CD192) is a seven-transmembrane, G-protein-coupled, glycoprotein receptor that belongs to the beta chemokine receptor family. It is expressed on basophils, monocytes/macrophages, dendritic cells, activated T cells and B cells. CCR2 (CD192) serves as a receptor for Monocyte chemoattractant protein 1 (MCP-1/CCL2), MCP-2/CCL8, MCP-3/CCL7, and MCP-4/CCL13. CD192 exists in two forms, CD192A and CD192B. The two forms are derived from alternatively spliced variants of a single gene and differ at their intracellular C-terminal ends. CD192 plays an important role in inflammatory responses including monocytic infiltration of tissues associated with certain diseases, eg, atherosclerosis, rheumatoid arthritis, and tumors.
The antibody was conjugated to BD Horizon™ BUV563 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 which has an Ex Max of 348 nm and an acceptor dye. The tandem has an Em Max at 563 nm. BD Horizon BUV563 can be excited by the 355 nm ultraviolet laser. On instruments with a 561 nm Yellow-Green laser, the recommended bandpass filter is 585/15 nm with a 535 nm long pass to minimize laser light leakage. When BD Horizon BUV563 is used with an instrument that does not have a 561 nm laser, a 560/40 nm filter with a 535 nm long pass may be more optimal. Due to the excitation and emission characteristics of the acceptor dye, there may be spillover into the PE and PE-CF594 detectors. However, the spillover can be corrected through compensation as with any other dye combination.