The 83103 monoclonal antibody specifically binds to CD193, which is also known as, C-C chemokine receptor type 3 (CCR3). CD193 is a chemokine receptor for CCL11/eotaxin, CCL24/eotaxin-2, CCL26/eotaxin-3, CCL5/RANTES, CCL7/MCP-3, CCL8/MCP-2, CCL14/MCP-4, CCL15/HCC-2 and CCL3/MIP-1a. CD193 is reportedly expressed on eosinophils, mast cells and Th2 cells. It plays an important role in eosinophil recruitment to the skin and the lung and in the development of airway hyperresponsiveness.
The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.