The 34-5-3 antibody reacts with the β chain of the I-A[d] MHC class II alloantigen. It cross-reacts with I-A[b] and with cells from mice of the H-2[p] and H-2[q] haplotypes. Reactivity with other haplotypes (e.g., a, f, k, r, s) has not been observed. The strain distribution of the antigen recognized by this reagent is similar to that of anti-I-A[b] mAb 25-9-17 (Cat. Nos. 553603, 553604, and 553605).
The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.