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    Biotin Rat Anti-Mouse Vα 8.3 T-Cell Receptor
    Biotin Rat Anti-Mouse Vα 8.3 T-Cell Receptor
    Expression of Va 8.3 TCR on peripheral T lymphocytes. BALB/c lymph node leukocytes were stained with PE-conjugated anti-mouse CD3e mAb 145-2C11 (Cat. no. 553063/553064) and either biotinylated rat IgG2a, k isotype control mAb R35-95 (Cat. no. 553928, Panel A) or biotinylated mAb KT50 (Panel B), followed by Avidin-FITC (Cat. no. 554060). Viable lymphocytes were selected by exclusion of 7-AAD (BD Via-Probe™, Cat. no. 555815/555816) and scatter profile. Flow cytometry was performed on a BD FACScan™ System (BD Biosciences, San Jose, CA).
    Biotin Rat Anti-Mouse Vα 8.3 T-Cell Receptor
    Expression of Va 8.3 TCR on peripheral T lymphocytes. BALB/c lymph node leukocytes were stained with PE-conjugated anti-mouse CD3e mAb 145-2C11 (Cat. no. 553063/553064) and either biotinylated rat IgG2a, k isotype control mAb R35-95 (Cat. no. 553928, Panel A) or biotinylated mAb KT50 (Panel B), followed by Avidin-FITC (Cat. no. 554060). Viable lymphocytes were selected by exclusion of 7-AAD (BD Via-Probe™, Cat. no. 555815/555816) and scatter profile. Flow cytometry was performed on a BD FACScan™ System (BD Biosciences, San Jose, CA).
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    BD Pharmingen™
    Mouse (QC Testing)
    Rat SD, also known as Sprague-Dawley (outbred) IgG2a, κ
    Mouse T-cell clone C6
    Flow cytometry (Routinely Tested)
    0.5 mg/ml
    AB_396583
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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    Recommended Assay Procedures

    For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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    557096 Rev. 3
    Antibody Details
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    KT50

    The KT50 antibody reacts with some members of the Vα 8 T-cell Receptor (TCR) subfamily of mice having the a, b, c, and d haplotypes of the Tcra gene complex (e.g., all strains tested). It recognizes an epitope in the CDR1 of Vα 8.3, but not Vα 8.2, TCR subfamily member, as does the B21.14 mAb (Cat. no. 553374). Site-directed mutagenesis has identified three amino acids which are necessary for antibody reactivity and which are unique to Vα 8.3 among the five functional Vα 8 TCR subfamily members. On a common H-2[k] background, the frequency of Vα 8.3 TCR-bearing T lymphocytes is higher in Tcra[a/a] mice than in Tcra[a/b] mice. Furthermore, studies of congenic strains suggest that CD8+ Vα 8.3 TCR-bearing T lymphocytes undergo negative selection in mice expressing MHC class I antigens of the H-2[d] haplotype.

    557096 Rev. 3
    Format Details
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    Biotin
    Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
    Biotin
    557096 Rev.3
    Citations & References
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    Development References (4)

    1. Brodnicki TC, Holman PO, Kranz DM. Reactivity and epitope mapping of single-chain T cell receptors with monoclonal antibodies. Mol Immunol. 1996; 33(3):253-263. (Biology). View Reference
    2. Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology). View Reference
    3. Tomonari K, Lovering E, Fairchild S, Spencer S. Two monoclonal antibodies specific for the T cell receptor V alpha 8. Eur J Immunol. 1989; 19(6):1131-1135. (Immunogen). View Reference
    4. Tomonari K. Negative selection of Tcra-V8+CD8+ T cells by MHC class I molecules. Immunogenetics. 1992; 35(5):291-295. (Biology). View Reference
    View All (4) View Less
    557096 Rev. 3

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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