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Biotin Rat Anti-Mouse Vα 3.2 [b,c] TCR
Biotin Rat Anti-Mouse Vα 3.2 [b,c] TCR

Two-color analysis of the expression of Vα 3.2[b,c] TCR on peripheral lymphocytes. C57BL/6 lymph node leukocytes were simultaneously stained with biotinylated RR3-16 (right panel), PE-conjugated anti-mouse CD4 RM4-5 (Cat. No. 553048/553049), and PE-conjugated anti-mouse CD8a 53-6.7 (Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

Two-color analysis of the expression of Vα 3.2[b,c] TCR on peripheral lymphocytes. C57BL/6 lymph node leukocytes were simultaneously stained with biotinylated RR3-16 (right panel), PE-conjugated anti-mouse CD4 RM4-5 (Cat. No. 553048/553049), and PE-conjugated anti-mouse CD8a 53-6.7 (Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

Product Details
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BD Pharmingen™
Mouse (QC Testing)
Rat F344, also known as Fischer, CDF IgG2b, κ
Mouse Cytolytic T-Cell Clone OH6
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_394714
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553218 Rev. 12
Antibody Details
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RR3-16

The RR3-16 monoclonal antibody specifically reacts with the Vα 3.2 T-Cell Receptor (TCR) of mice having the b (e.g., C57BL) and c (e.g., SWR, SJL, NZB, NOD) haplotypes of the Tcra gene complex, but not with TCR encoded by other members of Tcra-V3 gene subfamily. RR3-16 antibody does not react with strains having the a (e.g., A, AKR, BALB/c, CBA, C3H/He) or d (e.g., DBA/1, DBA/2, NZW) Tcra haplotypes. In addition, it has been shown that frequencies of Vα 3.2+ CD8+ T cells from homozygous H-2k/H-2k mice are moderately higher than those from heterozygous H-2k/H-2d mice, suggesting positive selection by H-2k.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

553218 Rev. 12
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
553218 Rev.12
Citations & References
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Development References (3)

  1. Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology). View Reference
  2. Utsunomiya Y, Bill J, Palmer E, Gollob K, Takagaki Y, Kanagawa O. Analysis of a monoclonal rat antibody directed to the alpha-chain variable region (V alpha 3) of the mouse T cell antigen receptor. J Immunol. 1989; 143(8):2602-2608. (Immunogen). View Reference
  3. Utsunomiya Y, Bill J, Palmer E, Kanagawa O. Identification of a mouse T-cell antigen receptor alpha-chain polymorphism by a V alpha 3.2 chain-specific monoclonal antibody. Immunogenetics. 1991; 33(3):198-201. (Clone-specific). View Reference
553218 Rev. 12

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.