Skip to main content Skip to navigation
Biotin Rat Anti-Mouse Vα 11.1, 11.2[b,d] TCR
Biotin Rat Anti-Mouse Vα 11.1, 11.2[b,d] TCR

Two-color analysis of the expression of Vα 11.1, 11.2 [b,d] TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotin-conjugated RR8-1 (right panel), PE-conjugated RM4-5 (anti-CD4, Cat. No. 553048/553049, both panels), and PE-conjugated 53-6.7 (anti-CD8a, Cat. No. 553032/553033, both panels) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057, both panels). Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).

Two-color analysis of the expression of Vα 11.1, 11.2 [b,d] TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotin-conjugated RR8-1 (right panel), PE-conjugated RM4-5 (anti-CD4, Cat. No. 553048/553049, both panels), and PE-conjugated 53-6.7 (anti-CD8a, Cat. No. 553032/553033, both panels) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057, both panels). Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).

Product Details
Down Arrow Up Arrow


BD Pharmingen™
Mouse (QC Testing)
Rat IgG2b, κ
Mouse T-Cell Clone B10
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_394716
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553221 Rev. 9
Antibody Details
Down Arrow Up Arrow
RR8-1

The RR8-1 monoclonal antibody specifically recognizes the Vα 11.1 and Vα 11.2, but not Vα 11.3 T-cell Receptors (TCR) of mice having the b (e.g., C57BL) and d (e.g., DBA/1, DBA/2, NZW) haplotypes of Tcra gene complex. RR8-1 antibody does not react with strains having the a (e.g., A, AKR, BALB/c, CBA, C3H/He) or c (e.g., NZB, SJL, SWR, NOD) Tcra haplotypes. Plate-bound RR8-1 antibody activates Vα 11.1, 11.2[b,d] TCR-bearing T lymphocytes.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

553221 Rev. 9
Format Details
Down Arrow Up Arrow
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
553221 Rev.9
Citations & References
Down Arrow Up Arrow

Development References (1)

  1. Jameson SC, Nakajima PB, Brooks JL, Heath W, Kanagawa O, Gascoigne NR. The T cell receptor V alpha 11 gene family. Analysis of allelic sequence polymorphism and demonstration of J alpha region-dependent recognition by allele-specific antibodies. J Immunol. 1991; 147(9):3185-3193. (Immunogen). View Reference
553221 Rev. 9

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.