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Biotin Rat Anti-Mouse I-A/I-E
Product Details
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BD Pharmingen™
H-2I; I-Ad, -Ed; I-Eb and I-Ek MHC class II alloantigens; Ia Ag; MHC II
Mouse (QC Testing)
Rat DA, also known as DA/HA IgG2a, κ
BALB/c mouse epidermal Langerhans cells
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
0.5 mg/ml
AB_394957
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

Other applications include immunohistochemical staining of acetone-fixed frozen sections.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553622 Rev. 14
Antibody Details
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2G9

The 2G9 monoclonal antibody reacts with the I-Ad and I-Ed MHC class II alloantigens.  It also reacts with transfectants expressing I-Eb and I-Ek and with cells from mice of the H-2p and H-2q haplotypes.  This antibody is non-reactive with cells from SJL (H-2s) and NOD (H-2g7) mice.  Flow cytometric analysis indicates that the 2G9 and M5/114.15.2 monoclonal antibodies have comparable reactivity on cells from mice with I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek alloantigens.

553622 Rev. 14
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
553622 Rev.14
Citations & References
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Development References (1)

  1. Becker D, Mohamadzadeh M, Reske K, Knop J. Increased level of intracellular MHC class II molecules in murine Langerhans cells following in vivo and in vitro administration of contact allergens. J Invest Dermatol. 1992; 99(5):545-549. (Immunogen). View Reference
553622 Rev. 14

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.