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Biotin Mouse anti-Mouse Ly-49F
Biotin Mouse anti-Mouse Ly-49F

Two-color analysis of Ly-49F xpression on splenic NK cells. C57BL/6 splenocytes were simultaneously  stained with APC-conjugated mAb PK136 (anti-mouse NK-1.1, Cat. no. 557391, both panels) and biotinylated mAb MOPC-31C (mouse IgG1, κ isotype control, Cat. no. 550615, Left Panel) or biotinylated mAb HBF-719 (Right Panel), followed by Streptavidin-PE (Cat. no. 554061, both panels). Flow cytometry was performed on a FACSCaliburª (BDIS, San Jose, CA).

Two-color analysis of Ly-49F xpression on splenic NK cells. C57BL/6 splenocytes were simultaneously  stained with APC-conjugated mAb PK136 (anti-mouse NK-1.1, Cat. no. 557391, both panels) and biotinylated mAb MOPC-31C (mouse IgG1, κ isotype control, Cat. no. 550615, Left Panel) or biotinylated mAb HBF-719 (Right Panel), followed by Streptavidin-PE (Cat. no. 554061, both panels). Flow cytometry was performed on a FACSCaliburª (BDIS, San Jose, CA).

Product Details
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BD Pharmingen™
Mouse (QC Testing)
Mouse IgG1, κ
CHO-K1 cells transfected with the B6 allele of the Ly-49F gene,1 Klra62 Transfected Cell Line
Flow cytometry (Routinely Tested)
0.5 mg/ml
16637
AB_393997
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
550986 Rev. 3
Antibody Details
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HBF-719

The HBF-719 antibody reacts with the B6 alloantigen of Ly-49F, an inhibitory receptor which is expressed on subsets of natural killer (NK) cells, NK-1.1+ (or DX5+) T lymphocytes (NK-T cells), and on a population of memory CD8+ T lymphocytes in C57BL/6 mice, 1 but not AKR/J, BALB/c, C3H/HeN, DBA/1, or SJL mice.3 The Ly-49 family of NK-cell receptors are disulfide linked type-II transmembrane protein homodimers with extracellular carbohydrate recognition domains (CRD). The Ly-49 family members are expressed independently, such that an individual NKor T-cell may display more than one class of Ly-49 receptor homodimers. Ly-49F has an ITIM (Immunoreceptor Tyrosine-based Inhibitory Motif) in its cytoplasmic tail. Therefore, it is probably an inhibitory receptor. It weakly binds to cells expressing the H-2d MHC class I alloantigens.

550986 Rev. 3
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
550986 Rev.3
Citations & References
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Development References (5)

  1. Coles MC, McMahon CW, Takizawa H, Raulet DH. Memory CD8 T lymphocytes express inhibitory MHC-specific Ly49 receptors. Eur J Immunol. 2000; 30(1):236-244. (Immunogen). View Reference
  2. Hanke T, Takizawa H, McMahon CW, et al. Direct assessment of MHC class I binding by seven Ly49 inhibitory NK cell receptors. Immunity. 1999; 11(1):67-77. (Biology). View Reference
  3. Raulet DH, Held W, Correa I, Dorfman JR, Wu MF, Corral L. Specificity, tolerance and developmental regulation of natural killer cells defined by expression of class I-specific Ly49 receptors. Immunol Rev. 1997; 155:41-52. (Biology). View Reference
  4. Takei F, Brennan J, Mager DL. The Ly-49 family: genes, proteins and recognition of class I MHC. Immunol Rev. 1997; 155:67-77. (Biology). View Reference
  5. The Jackson Laboratory. Mouse Genome Database (MGD), Mouse Genome Informatics Web Site. Available: http://www.informatics.jax.org September, 2003.
View All (5) View Less
550986 Rev. 3

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.