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Biotin Mouse Anti-Human CD124
Biotin Mouse Anti-Human CD124

Flow cytometric analysis of CD124 expression on human PBMC. Human PBMC isolated by density gradient centrifugation (Ficoll-Paque™) were blocked with either normal polyclonal human IgG and stained with Biotin Mouse Anti-Human CD124 (Cat. No. 552120; filled histogram) or Biotin Mouse IgG1, κ Isotype Control (Cat. No. 555747; open histogram), followed by PE Streptavidin (Cat. No. 554061). Fluorescence histograms depicting CD124 (or Ig isotype) expression were derived from gated events with the forward and side light-scatter characteristics of viable CD19+ lymphocytes.  

Note: Certain human cell lines or cell types (e.g., neutrophils and monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors.

Flow cytometric analysis of CD124 expression on human PBMC. Human PBMC isolated by density gradient centrifugation (Ficoll-Paque™) were blocked with either normal polyclonal human IgG and stained with Biotin Mouse Anti-Human CD124 (Cat. No. 552120; filled histogram) or Biotin Mouse IgG1, κ Isotype Control (Cat. No. 555747; open histogram), followed by PE Streptavidin (Cat. No. 554061). Fluorescence histograms depicting CD124 (or Ig isotype) expression were derived from gated events with the forward and side light-scatter characteristics of viable CD19+ lymphocytes.  

Note: Certain human cell lines or cell types (e.g., neutrophils and monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors.

Product Details
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BD Pharmingen™
IL-4 Receptor α Chain, CD124
Human (QC Testing)
Mouse IgG1, κ
Soluble Human IL-4 Receptor
Flow cytometry (Routinely Tested)
20 µl
V C004, BP169; VI BP205, C81
3566
AB_394339
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

Immunofluorescent Staining and Flow Cytometric Analysis: Biotin Mouse Anti-Human CD124 can be used for the immunofluorescent staining (20 µl/10^6 cells) and flow cytometric analysis of human nucleated cells to measure their expressed levels of surface CD124. An appropriate purified immunoglobulin isotype control is Biotin Mouse IgG1 κ Isotype Control (Cat. No. 555747).

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. Ficoll-Paque is a trademark of Amersham Biosciences Limited.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
552120 Rev. 2
Antibody Details
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hIL4R-M57

The hIL4R-M57 antibody specifically binds to the α subunit (IL-4Rα) of the human Interleukin-4 Receptor complex which is also known as CD124. The human IL-4Rα, also known as B cell stimulatory factor 1 receptor (BSF-1 receptor), is a 140 kDa transmembrane glycoprotein that is expressed by B and T lymphocytes and a variety of other hematopoietic and non-hematopoietic cells and cell lines. The cell surface IL-4Rα chain binds IL-4 with high affinity and associates with either the common γ chain (IL-4Rα/γc; aka, type I IL-4R complex) or the IL-13 receptor alpha-1 subunit (IL-4Rα/IL-13Rα1; aka, type II IL-4R complex) to form two distinct types of signal-transducing IL-4R complexes. The type I IL-4 receptor complex specifically binds IL-4 whereas the type II IL-4R complex binds and transduces signals from either IL-4 or IL-13. A truncated form of the IL-4Rα exists in soluble form in biological fluids. In contrast to mice, in humans no distinct mRNA coding for sIL-4R has been described, suggested that human sIL4-R is exclusively produced by proteolytic cleavage of the cell surface receptor. The serum levels of soluble IL-4Rα appear to elevate in pathological situations such as allergy and parasitic infections. Depending on the ratios of IL-4 and sIL-4Rα present in the local milieu, the sIL-4Rα may augment or antagonize the activities of IL-4. The immunogen used to generate the hIL4R-M57 hybridoma was soluble human IL-4R.

552120 Rev. 2
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
552120 Rev.2
Citations & References
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Development References (19)

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  7. Idzerda RL, March CJ, Mosley B, et al. Human interleukin 4 receptor confers biological responsiveness and defines a novel receptor superfamily. J Exp Med. 1990; 171(3):861-873. (Clone-specific). View Reference
  8. Jung T, Bews JP, Enssle KH, Wagner K, Neumann C, Heusser CH. Detection of and discrimination between total and free human interleukin-4 and free soluble interleukin-4 receptor by ELISA. J Immunol Methods. 1998; 217(1-2):41-50. (Biology). View Reference
  9. Jung T, Schrader N, Hellwig M, Enssle KH, Neumann C. Soluble human interleukin-4 receptor is produced by activated T cells under the control of metalloproteinases. Int Arch Allergy Immunol. 1999; 119(1):23-30. (Biology). View Reference
  10. Law CL, Armitage RJ, Villablanca JG, LeBien TW. Expression of interleukin-4 receptors on early human B-lineage cells. Blood. 1991; 78(3):703-710. (Biology). View Reference
  11. Mozo L, Rivas D, Zamorano J, Gutierrez C. Differential expression of IL-4 receptors in human T and B lymphocytes. J Immunol. 1993; 150(10):4261-4269. (Biology). View Reference
  12. Nasert S, Millner M, Enssle KH, Wahn U, Renz H. Differential modulation of T cell functions by soluble IL-4R (s1L-4R) in two cases of severe atopic dermatitis. Pediatr Allergy Immunol. 1996; 7(2):91-94. (Biology). View Reference
  13. Ohara J, Paul WE. Receptors for B-cell stimulatory factor-1 expressed on cells of haematopoietic lineage. Nature. 1987; 325(6104):537-540. (Biology). View Reference
  14. Park LS, Friend D, Sassenfeld HM, Urdal DL. Characterization of the human B cell stimulatory factor 1 receptor. J Exp Med. 1987; 166(2):476-488. (Biology). View Reference
  15. Sang DK, Ouma JH, John CC, et al. Increased levels of soluble interleukin-4 receptor in the sera of patients with visceral leishmaniasis. J Infect Dis. 1999; 179(3):743-746. (Biology). View Reference
  16. Zola H, Flego L, Weedon H. Expression of IL-4 receptor on human T and B lymphocytes. Cell Immunol. 1993; 150(1):149-158. (Clone-specific: Flow cytometry). View Reference
  17. Zuber CE, Galizzi JP, Harada N, Durand I, Banchereau J. Interleukin-4 receptors on human blood mononuclear cells. Cell Immunol. 1990; 129(2):329-340. (Biology). View Reference
  18. Zuber CE, Galizzi JP, Valle A, Harada N, Howard M, Banchereau J. Interleukin 4 receptors on normal human B lymphocytes: characterization and regulation. Eur J Immunol. 1990; 20(3):551-555. (Biology). View Reference
  19. Zurawski SM, Chomarat P, Djossou O, et al. The primary binding subunit of the human interleukin-4 receptor is also a component of the interleukin-13 receptor. J Biol Chem. 1995; 270(23):13869-13878. (Biology). View Reference
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552120 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.