Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Middle East / Africa (English)
Products

Link Arrow
Solutions

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
    Alert Icon
    APC Mouse Anti-Human CD65
    APC Mouse Anti-Human CD65

    Multiparameter flow cytometric analysis of CD65 expression on Human peripheral blood leukocyte populations.  Human whole blood was first treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes, washed, and then preincubated with BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/564220). The leukocytes were then stained with either APC Mouse IgM, κ Isotype Control (Cat. No. 550883; Left Plot) or APC Mouse Anti-Human CD65 antibody (Cat. No. 569470/569471). The bivariate pseudocolor density plot showing the correlated expression CD65 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

    APC Mouse Anti-Human CD65

    Multiparameter flow cytometric analysis of CD65 expression on Human peripheral blood leukocyte populations.  Human whole blood was first treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes, washed, and then preincubated with BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/564220). The leukocytes were then stained with either APC Mouse IgM, κ Isotype Control (Cat. No. 550883; Left Plot) or APC Mouse Anti-Human CD65 antibody (Cat. No. 569470/569471). The bivariate pseudocolor density plot showing the correlated expression CD65 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

    Product Details
    Down Arrow Up Arrow


    BD Pharmingen™
    Fucoganglioside Type II: Ceramide dodecasacharide 4c
    Human (QC Testing)
    Mouse IgM, κ
    Human THP-1 Cell Line
    Flow cytometry (Routinely Tested)
    5 µl/test
    IV M23; V MA95
    AB_3685085
    Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
    Show More blue down arrow Show Less blue up arrow

    Recommended Assay Procedures

    BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

    Show More blue down arrow Show Less blue up arrow

    Product Notices

    1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    2. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
    3. An isotype control should be used at the same concentration as the antibody of interest.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
    6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    7. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
    9. For U.S. patents that may apply, see bd.com/patents.
    Show More blue down arrow Show Less blue up arrow
    569470 Rev. 1
    Antibody Details
    Down Arrow Up Arrow
    VIM8

    The VIM8 monoclonal antibody specifically binds to the asialylated form of human CD65. CD65 is also known as, Ceramide dodecasacharide 4c. CD65 is expressed on myeloid cells during development. Peripheral blood granulocytes express high levels of CD65when compared with monocytes that express lower levels. CD65 reportedly can serve as a ligand for CD62E (E-Selectin). CD65 has been implicated as a crucial adhesive ligand involved in the extravascular infiltration of acute myeloid leukemic (AML) cells.

    569470 Rev. 1
    Format Details
    Down Arrow Up Arrow
    APC
    Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    APC
    Red 627-640 nm
    651 nm
    660 nm
    569470 Rev.1
    Citations & References
    Down Arrow Up Arrow
    View product citations for antibody "569470" on CiteAb

    Development References (7)

    1. Easton EW, Schiphorst WE, van Drunen E, van der Schoot CE, van den Eijnden DH. Human myeloid alpha 3-fucosyltransferase is involved in the expression of the sialyl-Lewis(x) determinant, a ligand for E- and P-selectin. Blood. 1993; 81(11):2978-2986. (Clone-specific: Flow cytometry). View Reference
    2. Gooi HC, Hounsell EF, Edwards A, Majdic O, Knapp W, Feizi T. Differences in the fine specificities of monoclonal (Class A) antibodies to human myeloid cells.. Clin Exp Immunol. 1985; 60(1):151-8. (Immunogen: Immunofluorescence, Radioimmunoassay). View Reference
    3. Knapp W., Majdic O, Blanchard D, et al. CDw65 cluster workshop report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:876-882.
    4. Kniep B, Peter-Katalinic J, Müthing J, Majdic O, Pickl WF, Knapp W. The CDw65 monoclonal antibodies VIM-8 and VIM-11 bind to the neutral glycolipid V3FucnLc8Cer.. J Biochem. 1996; 119(3):456-62. (Clone-specific: Flow cytometry, Western blot). View Reference
    5. Noguchi M, Sato N, Sugimori H, Mori K, Oshimi K. A minor E-selectin ligand, CD65, is critical for extravascular infiltration of acute myeloid leukemia cells.. Leuk Res. 2001; 25(10):847-53. (Biology). View Reference
    6. Paietta E, Neuberg D, Bennett JM, et al. Low expression of the myeloid differentiation antigen CD65s, a feature of poorly differentiated AML in older adults: study of 711 patients enrolled in ECOG trials.. Leukemia. 2003; 17(8):1544-50. (Biology). View Reference
    7. Stockinger H. Cluster report: CDw65. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:836-838.
    View All (7) View Less
    569470 Rev. 1

    Please refer to Support Documents for Quality Certificates

     

    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

     

    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.