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APC Goat Anti-Rat Ig

APC Goat Anti-Rat Ig

Clone Polyclonal

(RUO)
Product Details
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BD Pharmingen™
Rat (QC Testing)
Goat Ig
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_398484
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

The purified antibody solution was passed through solid-phase immunoadsorbent gels to minimize cross-reactivity with mouse, human, cow, and horse serum proteins.

Recommended Assay Procedures

This antibody stains rat peripheral B cells, and it has little reactivity with rat non-B splenocytes or mouse splenocytes. As a second step, it is reactive with rat IgG and IgM monoclonal antibodies; a weaker signal is detected when the primary antibody has a rat IgG2b isotype. It has weak cross-reactivity detectable by flow cytometry with some, but not all, hamster immunoglobulins. Consequently, it may be useful as a primary reagent in immunofluorescent staining of rat antibody-producing cells or as a secondary reagent for staining of mouse leukocytes after reaction with rat Ig primary antibodies. However, we have observed that the reactivity of polyclonal second-step antibodies to mouse or rat IgM may be reduced after adsorption against Ig of rat or mouse, respectively. Because this anti-rat Ig antibody was adsorbed with mouse Ig, it may be weakly reactive with some rat IgM primary antibodies.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
551019 Rev. 6
Antibody Details
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Poly1271
551019 Rev. 6
Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC
Red 627-640 nm
651 nm
660 nm
551019 Rev.6

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.