Flow cytometric staining profile of Alexa Fluor® 647 Rat Anti-Mouse CD193 on mouse CCR3- transfected L1.2 cells. Mouse CCR3-transfected L1.2 cells (open histogram) or wild type L1.2 cells (shaded histogram) were stained with Alexa Fluor ® 647 Rat Anti-Mouse CD193 antibody (clone 83103; Cat. No. 557974).
Flow cytometric staining profile of Alexa Fluor® 647 Rat Anti-Mouse CD193 on mouse CCR3- transfected L1.2 cells. Mouse CCR3-transfected L1.2 cells (open histogram) or wild type L1.2 cells (shaded histogram) were stained with Alexa Fluor ® 647 Rat Anti-Mouse CD193 antibody (clone 83103; Cat. No. 557974).
Product Details
BD Pharmingen™
CCR3; CCR-3; chemokine (C-C) receptor 3; CC-CKR3; C-C CKR-3; CKR3; CC-CKR-3
Mouse (QC Testing)
Rat IgG2a
Mouse CCR3 transfected Cells Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_396967
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Lysing Solution 10X Concentrate CE_IVD
Size
100 mL
Cat No.
349202
Alexa Fluor® 647 Rat IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
557690
557974 Rev. 4
Antibody Details
83103
The 83103 monoclonal antibody specifically binds to CD193, which is also known as, C-C chemokine receptor type 3 (CCR3). CD193 is a chemokine receptor for CCL11/eotaxin, CCL24/eotaxin-2, CCL26/eotaxin-3, CCL5/RANTES, CCL7/MCP-3, CCL8/MCP-2, CCL14/MCP-4, CCL15/HCC-2 and CCL3/MIP-1a. CD193 is reportedly expressed on eosinophils, mast cells and Th2 cells. It plays an important role in eosinophil recruitment to the skin and the lung and in the development of airway hyperresponsiveness.
557974 Rev. 4
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
557974 Rev.4
Citations & References
Development References (2)
-
Ma W, Bryce PJ, Humbles AA. CCR3 is essential for skin eosinophilia and airway hyperresponsiveness in a murine model of allergic skin inflammation. J Clin Invest. 2002; 109(5):621-628. (Biology). View Reference
-
Teixeira MM, Wells TN, Lukacs NW. Chemokine-induced eosinophil recruitment. Evidence of a role for endogenous eotaxin in an in vivo allergy model in mouse skin. J Clin Invest. 1997; 100(7):1657-1666. (Biology). View Reference
557974 Rev. 4
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
