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Alexa Fluor® 647 Mouse Anti-Human CD309 (VEGFR-2)
Alexa Fluor® 647 Mouse Anti-Human CD309 (VEGFR-2)

Flow cytometric analysis of Alexa Fluor® 647 anti-human VEGFR-2/KDR on HUVEC cells. HUVEC (Lonza, Cat. No. CC-2517) cells grown in EGM®-2 Endothelial Cell Growth Medium (Lonza, Cat No. CC-3162), which contains VEGF, were dissociated with Cell Dissociation Buffer (Life Technologies, Cat. No. 13151-014).  The HUVEC cells were stained with either Alexa Fluor® 647 anti-human VEGFR-2/KDR (solid line) or Alexa Fluor® 647 mouse IgG1 k Isotype Control (Clone MOPC-21, Cat. No. 557714) (dotted line). Flow cytometry was performed on a BD™ LSR II flow cytometry system.

Flow cytometric analysis of Alexa Fluor® 647 anti-human VEGFR-2/KDR on HUVEC cells. HUVEC (Lonza, Cat. No. CC-2517) cells grown in EGM®-2 Endothelial Cell Growth Medium (Lonza, Cat No. CC-3162), which contains VEGF, were dissociated with Cell Dissociation Buffer (Life Technologies, Cat. No. 13151-014).  The HUVEC cells were stained with either Alexa Fluor® 647 anti-human VEGFR-2/KDR (solid line) or Alexa Fluor® 647 mouse IgG1 k Isotype Control (Clone MOPC-21, Cat. No. 557714) (dotted line). Flow cytometry was performed on a BD™ LSR II flow cytometry system.

Product Details
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BD Pharmingen™
FLK1; Fetal liver kinase 1; KDR; VEGFR2; VEGF Receptor 2
Human (QC Testing)
Mouse IgG1
Human VEGFR-2
Flow cytometry (Routinely Tested)
20 µl
AB_1645404
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
560871 Rev. 2
Antibody Details
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89106

The 89106 monoclonal antibody reacts with CD309 (vascular endothelial growth factor receptor-2 (VEGFR-2)), a receptor protein tyrosine kinase closely related to CD117 (c-kit) and CD140a (PDGF Receptor α chain) of the immunoglobulin superfamily. VEGFR-2, also known as fetal liver kinase 1 (Flk-1) or kinase insert domain receptor (KDR), is a receptor for vascular endothelial growth factor (VEGF). It is expressed, at the mRNA and protein levels, on distinct sets of mesoderm during gastrulation and on endothelial cells in embryonic tissues.   In vivo and in vitro studies indicate that VEGFR-2 is required for the embryonic development of vascular endothelial and hematopoietic cells. Human cardiac progenitor cells derived from human embryonic stem cells arise from a population of cells that express VEGFR-2.

560871 Rev. 2
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
560871 Rev.2
Citations & References
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Development References (4)

  1. Farace F, Massard C, Borghi E, Bidart JM, Soria JC. Vascular disrupting therapy-induced mobilization of circulating endothelial progenitor cells. Ann Oncol. 2007; 18(8):1421-1422. (Clone-specific: Flow cytometry). View Reference
  2. Ferrara N, Houck K, Jakeman L, Leung DW. Molecular and biological properties of the vascular endothelial growth factor family of proteins. Endocr Res. 1992; 13(1):18-32. (Biology). View Reference
  3. Yang L, Soonpaa MH, Adler ED, et al. Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population. Nature. 2008; 453(7194):524-528. (Clone-specific: Flow cytometry). View Reference
  4. Ziegler BL, Valtieri M, Porada GA, et al. KDR receptor: a key marker defining hematopoietic stem cells. Science. 1999; 285:1553-1558. (Biology). View Reference
View All (4) View Less
560871 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.