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FITC Mouse Anti-Human Granzyme A Reagent Set(RUO)
Profile of permeabilized peripheral blood lymphocytes analyzed by flow cytometry (BDIS, San Jose, CA). Cells were collected, fixed, and permeabilized using the Cytofix/Cytoperm™ Kit (Cat. No. 554715) for 20 minutes at room temperature (RT), pelleted and washed twice with Perm/Wash Buffer™. Cells were resuspended in Perm/Wash Buffer and stained with FITC anti-human Granzyme A (clone CB9, Cat. No. 558905) or with an isotype control (clone MOPC-21) for 20-30 minutes at RT in the dark. Cells were then washed once in Perm/Wash Buffer, resuspended in wash buffer and analyzed by flow cytometry.
BD Pharmingen™ FITC Mouse Anti-Human Granzyme A Reagent Set
FITC Mouse Anti-Human Granzyme A Reagent Set
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