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FITC Mouse Anti-Human Cyclin D1 Antibody Set

FITC Mouse Anti-Human Cyclin D1 Antibody Set

(RUO)
FITC Mouse Anti-Human Cyclin D1 Antibody Set
Profile of WI-38 human diploid fibroblasts analyzed on a FACScan™ (BDIS, San Jose, CA). Cells were stained with anti-human Cyclin D1-FITC (51-13864X) or a mouse IgG1-FITC  isotype (negative) control (51-13854X-2). DNA was stained with propidium iodide.
Profile of WI-38 human diploid fibroblasts analyzed on a FACScan™ (BDIS, San Jose, CA). Cells were stained with anti-human Cyclin D1-FITC (51-13864X) or a mouse IgG1-FITC  isotype (negative) control (51-13854X-2). DNA was stained with propidium iodide.
Product Details
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BD Pharmingen™
Human (QC Testing)
Flow cytometry (Routinely Tested)
RUO
AB_395246


Description

Cyclins and cyclin-dependent kinases (cdks) are subunits of cell cycle dependent protein kinases that regulate key events during the progression of the cell cycle. Specific substrates for cdk/cyclin kinases include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large-T), tumor suppressor genes (the  retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. Human cyclin D1 migrates at a molecular weight of 36 kDa in SDS/PAGE.

Clone G124-326 recognizes human Cyclin D1. It does not cross-react with human cyclins D2 and D3. Recombinant full-length human Cyclin D1 was used as immunogen. The G124-326 antibody was initially characterized by immunoprecipitation and western blot analysis. In publications it has been used for western blot analysis, flow cytometric analysis, and immunohistochemistry of frozen tissue sections.

Clone MOPC-21 is a mouse IgG1 isotype (negative) control. The MOPC-21 antibody has unknown specificity. The G124-326 and MOPC-21 FITC conjugates are matched in F/P ratios. The optimum F/P ratio was experimentally determined by flow cytometric analysis.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

Flow cytometry: WI-38 human fibroblasts (ATCC CCL-75) are suggested as a positive control.

For use in Western blot analysis, the Cyclin D1 antibody, clone G124-326, is also available in a purified format (Cat. No. 554180; 0.1 mg and No. 554181; 0.25 mg).

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. This antibody has been optimized and preassayed with its matched isotype control to be used at the recommended volume of 20 ul/test. Titration of the reagents or substituting with other (non-matched) isotype control is NOT recommended.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
554109 Rev. 9
Components
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Description Quantity/Size Part Number EntrezGene ID
FITC Mouse Anti-Human Cyclin D1 100 Tests (1 ea) 51-13864X N/A
FITC Mouse IgG1, kappa Isotype Control 100 Tests (1 ea) 51-13854X N/A
554109 Rev. 9
Citations & References
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Development References (5)

  1. Darzynkiewicz Z, Gong J, Juan G, Ardelt B, Traganos F. Cytometry of cyclin proteins. Cytometry. 1996; 25(1):1-13. (Clone-specific: Flow cytometry).
  2. Gong J, Traganos F, Darzynkiewicz Z. Threshold expression of cyclin E but not D type cyclins characterizes normal and tumour cells entering S phase. Cell Prolif. 1995; 28(6):337-346. (Clone-specific: Flow cytometry, Western blot). View Reference
  3. Gorospe M, Liu Y, Xu Q, Chrest FJ, Holbrook NJ. Inhibition of G1 cyclin-dependent kinase activity during growth arrest of human breast carcinoma cells by prostaglandin A2. Mol Cell Biol. 1996; 16(3):762-770. (Clone-specific: Western blot).
  4. Shapiro GI, Edwards CD, Kobzik L, et al. Reciprocal Rb inactivation and p16INK4 expression in primary lung cancers and cell lines. Cancer Res. 1995; 55(3):505-509. (Clone-specific: Immunohistochemistry, Western blot). View Reference
  5. Sherr CJ. Mammalian G1 cyclins. Cell. 1993; 73(6):1059-1065. (Biology). View Reference
554109 Rev. 9

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.